Primary Information |
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| BoMiProt ID | Bomi8422 |
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| Protein Name | Protein SMG8/Protein smg-8 homolog |
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| Organism | Bos taurus |
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| Uniprot ID | A1A4J7 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001073755.1 |
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| Aminoacid Length | 999 |
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| Molecular Weight | 110135 |
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| FASTA Sequence |
Download |
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| Gene Name | SMG8 |
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| Gene ID | 522040 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | take part in Nonsense-mediated mRNA decay (NMD) of mRNAs containing premature stop codons. |
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| Biochemical Properties | SMG8 has a C-terminal kinase inhibitory domain (KID), which covers the catalytic pocket and inhibits the kinase activity of SMG1. SMG8 and SMG9 form a pseudo symmetric dimer and the interaction is mediated by extensive hydrophobic contacts.Residues L274, L279 and H297 of SMG9 Switch II bind to residues F219, I221, D224 and I311 of SMG8. |
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| PTMs | Methylation and phosphorylation on Ser |
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| Linking IDs | Bomi8422 |
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| Bibliography | Zhu L, Li L, Qi Y, Yu Z, Xu Y. Cryo-EM structure of SMG1-SMG8-SMG9 complex. Cell Res. 2019 Dec;29(12):1027-1034. doi: 10.1038/s41422-019-0255-3. Epub 2019 Nov 15. PMID: 31729466; PMCID: PMC6951342. |
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| Protein Function | take part in Nonsense-mediated mRNA decay (NMD) of mRNAs containing premature stop codons. |
|---|
| Biochemical Properties | SMG8 has a C-terminal kinase inhibitory domain (KID), which covers the catalytic pocket and inhibits the kinase activity of SMG1. SMG8 and SMG9 form a pseudo symmetric dimer and the interaction is mediated by extensive hydrophobic contacts.Residues L274, L279 and H297 of SMG9 Switch II bind to residues F219, I221, D224 and I311 of SMG8. |
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| PTMs | Methylation and phosphorylation on Ser |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A1A4J7|SMG8_BOVIN Nonsense-mediated mRNA decay factor SMG8 OS=Bos taurus OX=9913 GN=SMG8 PE=2 SV=2
MAGPVSLRELLMGASAWTSSESPEGSPTEGGGSAAGGPEPPWREDEICVVGIFGKTALRL
NSEKFSLVNTVCDRQVFPLFRHQDPGDSGAGIRTEAGAVGEAGGAGDPGAGAGAGAGAGA
GDPVRGGVTAAEGNRTEPGSQDYSLLQAYYNQESKVLYLLLTSICDNSQLLRACRALQSG
EAGGGLSLPHAEAHEFWKHQEKVQCLSLLYLFSVCHILLLVHPTCSFDITYDRVFRALDG
LRQKVLPLLKTAIKDCPVGKDWKLNCRPCPPRLLFLFQLNGALKVEPPRNQDPAHPDKPK
KHSPKRRLQHALEDQIYRIFRKSRVLTNQSINCLFTVPANQAFVYIVPGSQEEDPVGMLL
DQLKSHCTVKDPESLLVPAPLSGSRRYQVMRQHSRQQLSFHTDTSSSSSSGQLVDFTLRE
FLWQHVELVLSKKGFDDSVGRNPQPSHFELPTYQKWISAASKLYEVAIDGKEEDPAS*477PTG
ELTSKILSSIKVLEGFLDIDTKFSENRCQKALPMAHSAYQSNLPHNYTMTVHKNQLAQAL
RVYSQHARGPAFHKYAMQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHSLPKSGEKPEA
DRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAANYDFYQLLEEKCCGKLDHINFPV
FEPSTPDPAPAKNESS*676PAPPDADADKLKEKEPQTQGESTSLSLALSLGQSTDSLGTYPAD
PQAGGDNPEVHGQGEVKTEKRPNLVDRQAS*750TVEYLPGMLHSNCPKGLLPKFSSWSLVKLG
PAKSYNFHTGLDQQGFIPGTNYLMPWDIVIRTRAEDEGDLDTNSWPAPNKAVPGKRSAVV
MGRGRRRDDIARAFVGFEYEDSRGRRFMCSGPDKVMKVMGSGPKESALKALNSDMPLYIL
SSS*903QGRGLKPHYAQLMRLFVVVPDAPLQIILTPQVRPGPPPCPVFYPEKQEITLPPDGLW
VLRFPYAYVTERGPCFPPKENVQLMSYKVLRGVLKAVTQ
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| Predicted Disorder Regions | 18-42, 87-95, 114-138, 290-306, 675-735, 837-843 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Linking IDs | |
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| Bibliography | Zhu L, Li L, Qi Y, Yu Z, Xu Y. Cryo-EM structure of SMG1-SMG8-SMG9 complex. Cell Res. 2019 Dec;29(12):1027-1034. doi: 10.1038/s41422-019-0255-3. Epub 2019 Nov 15. PMID: 31729466; PMCID: PMC6951342. |
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