Primary Information |
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| BoMiProt ID | Bomi7127 |
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| Protein Name | Microtubule-associated protein 4/MAP-4/Microtubule-associated protein U/MAP-U |
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| Organism | Bos taurus |
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| Uniprot ID | P36225 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_776530.1 |
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| Aminoacid Length | 1072 |
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| Molecular Weight | 111915 |
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| FASTA Sequence |
Download |
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| Gene Name | MAP4 |
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| Gene ID | 281295 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Promotes microtubule assembly. |
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| PTMs | Acetylation, Isopeptide bond formation, Phosphorylation on Ser and Thr, Ubl conjugation |
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| Significance of PTMs | Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules.Phosphorylation on Ser-734 negatively regulates MAP4 activity to promote microtubule assembly.In humans, the critical phosphorylation sites responsible for MAP4 detachment from MTs are S768, S787 and S696 (the corresponding sites in mice are predicted to be S737, S760 and S667). p38/MAPK activation could lead to MAP4 phosphorylation with MT disassembly in cardiomyocytes during hypoxia . |
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| Linking IDs | Bomi7127 |
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| Bibliography | Zhang J, Li L, Zhang Q, Wang W, Zhang D, Jia J, Lv Y, Yuan H, Song H, Xiang F, Hu J, Huang Y. Microtubule-associated protein 4 phosphorylation regulates epidermal keratinocyte migration and proliferation. Int J Biol Sci. 2019 Jul 24;15(9):1962-1976. doi: 10.7150/ijbs.35440. PMID: 31523197; PMCID: PMC6743305. |
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| Protein Function | Promotes microtubule assembly. |
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| PTMs | Acetylation, Isopeptide bond formation, Phosphorylation on Ser and Thr, Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|P36225|MAP4_BOVIN Microtubule-associated protein 4 OS=Bos taurus OX=9913 GN=MAP4 PE=1 SV=1
MADLS*5LADALTEPS*14PEIEEEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGSS*60
ESKKKPCSDTSQVEGTPSSQAAVLANGDHGIERNDVTGFPSEFLEEKMAYQGYQNSQNWP
ENTNFCFEPEHMVNPIQTDPFKMHHDDGLEDSLFFPSGTTNTSVFVEQNDPLKDTYGMLP
CDTFAPAAVVPQGWSAEAPNLAHLESFTSPEAVPQPLQPTAEPAEKVEMASGEERAEALE
MMMKLQAADMAPS*253REAEMVLAKDVVPATETGVALAKDMES*280PT*282KPDEALVKDVESSIESDM
ALVKDVVLPVQTEETAVKDAILPTETDVSLDEDLALSTETEVSTAQDILLFKET*354ESIPPV
TMDLASAEGTVPPTDQEMTPVKVAAS*386LSEIEAPLDEDIVSSTEIRSAKEIGLSS*414ET*416EVAL
SREMGLPPETEAILDKDMAAPPETEVIVPVKDMAPSPGIETTLAKDVAPRQEIEVTLGKD
TVSLPATEMALGRNVALPPET*501EVTLAKDVAQPPETEVNLANNAALAKFS*529EAEVVPVPVKD
METAQTQEATSEDSQLKSLQDEGQSAVPLMTS*572PEAVVAMGQKHS*584LPTDEDS*591VLEELEQKK
PSSQTSELPSETSGVAKPEEGPPTGSVSGNDITAPPNKELPPS*643PEKKTKPLATTQPAKT*660S
TSKAKTQPT*670SLPKQTAPTTLGGSNKKPMSLASGSVPAAPPKRPAAATSRPSTLPSKDTKP
KPVAEAKIPEKRVS*734PSKPASAPAVKPGSKSTQAVPKAPATATLASPGSTSRNLS*774TPLPKR
PTAIKTEGKPAEIKKMATKS*800APADLSRPKSTTTSSVKKSTTVPGTAPPAGAPSRARPTAT
PPRPSGTPPVDKKPTAAKPTSSAPRLGRVAANAS*874APDLKNVRSKVGS*887T*888ENIKHQPGGGRA
KVEKKTEAAAPARKPEPNAVTKAAGPIGNAQKPPTGKVQIQNKKVDISKVSSKCGSKANI
KHKPGGGDVKIESQKLNFKEKAQAKVGS*988LDNVGHLPAGGAVKTEGGGSEAPPCPGPPAGE
ELAIPEAAPEAGAPASASGLSGHTTLAGGGDQREAQTLDS*1060QIQETS*1066KWLGLA
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| Predicted Disorder Regions | 7-33, 58-69, 192-220, 250-267, 296-417, 454-619, 654-657, 709-753, 799-817, 855-903, 940-993, 1022-1068 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules.Phosphorylation on Ser-734 negatively regulates MAP4 activity to promote microtubule assembly.In humans, the critical phosphorylation sites responsible for MAP4 detachment from MTs are S768, S787 and S696 (the corresponding sites in mice are predicted to be S737, S760 and S667). p38/MAPK activation could lead to MAP4 phosphorylation with MT disassembly in cardiomyocytes during hypoxia . |
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| Linking IDs | |
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| Bibliography | Zhang J, Li L, Zhang Q, Wang W, Zhang D, Jia J, Lv Y, Yuan H, Song H, Xiang F, Hu J, Huang Y. Microtubule-associated protein 4 phosphorylation regulates epidermal keratinocyte migration and proliferation. Int J Biol Sci. 2019 Jul 24;15(9):1962-1976. doi: 10.7150/ijbs.35440. PMID: 31523197; PMCID: PMC6743305. |
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