Primary Information |
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| BoMiProt ID | Bomi3721 |
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| Protein Name | Adhesion G protein-coupled receptor L3/Latrophilin-3 |
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| Organism | Bos taurus |
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| Uniprot ID | O97827 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_851376.1 |
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| Aminoacid Length | 1580 |
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| Molecular Weight | 176051 |
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| FASTA Sequence |
Download |
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| Gene Name | ADGRL3/LPH3/LPHN3 |
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| Gene ID | 282651 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Presence in other biological fluids/tissue/cells | Brain-specific distribution but low levels are also detected in lung and spleen. |
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| Protein Function | Take part in cell-cell interactions through their enormous extracellular N-terminal domain and intracellular signaling via their canonical heptahelical transmembrane domain. |
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| Biochemical Properties | GPCR is cleaved at the conserved GPCR autoproteolysis site (GPS), but the resulting N-terminal fragment (NTF) and C-terminal fragment (CTF) remain attached to each other and incorporate into the plasma membrane as a non-covalently bound unit.NTF acts to suppress the intrinsic activity of the CTF.The peptide segment immediately following the GPS is critical for enhanced signaling, suggesting that this peptide stretch (also known as the tethered agonist (TA), Stachel, or stalk peptide) acts as an internal agonist in a manner analogous to that of protease-activated GPCRs (PARs). |
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| PTMs | O-glycosylated (major) and N-glycosylated,proteolytic cleavage |
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| Significance of PTMs | Proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. |
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| Linking IDs | Bomi3721 |
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| Bibliography | Mathiasen S, Palmisano T, Perry NA, Stoveken HM, Vizurraga A, McEwen DP, Okashah N, Langenhan T, Inoue A, Lambert NA, Tall GG, Javitch JA. G12/13 is activated by acute tethered agonist exposure in the adhesion GPCR ADGRL3. Nat Chem Biol. 2020 Dec;16(12):1343-1350. doi: 10.1038/s41589-020-0617-7. Epub 2020 Aug 10. Erratum in: Nat Chem Biol. 2020 Aug 17;: PMID: 32778842; PMCID: PMC7990041. |
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| Presence in other biological fluids/tissue/cells | Brain-specific distribution but low levels are also detected in lung and spleen. |
|---|
| Protein Function | Take part in cell-cell interactions through their enormous extracellular N-terminal domain and intracellular signaling via their canonical heptahelical transmembrane domain. |
|---|
| Biochemical Properties | GPCR is cleaved at the conserved GPCR autoproteolysis site (GPS), but the resulting N-terminal fragment (NTF) and C-terminal fragment (CTF) remain attached to each other and incorporate into the plasma membrane as a non-covalently bound unit.NTF acts to suppress the intrinsic activity of the CTF.The peptide segment immediately following the GPS is critical for enhanced signaling, suggesting that this peptide stretch (also known as the tethered agonist (TA), Stachel, or stalk peptide) acts as an internal agonist in a manner analogous to that of protease-activated GPCRs (PARs). |
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| PTMs | O-glycosylated (major) and N-glycosylated,proteolytic cleavage |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|O97827|AGRL3_BOVIN Adhesion G protein-coupled receptor L3 OS=Bos taurus OX=9913 GN=ADGRL3 PE=2 SV=1
MWPSQLLVFMMLLAPIIHGGKHSERHPALASPLRHAERGPGGALPPRHLLQQPAAERATA
HRGPGPRGATRGVRGPGAHGAQISAQAFSRAPIPMAVVRRELSCESYPIELRCPGTDVIM
IESANYGRTDDKICDSDPAQMENIRCYLPDAYKIMSQRCNN*161RTQCAVVAGPDVFPDPCPG
TYKYLEVQYECVPYKVEQKVFLCPGLLKGVYQSEHLFESDHQSGAWCKDPLQASDKIYYM
PWTPYRTDTLTEYSSKDDFIAGRPTTTYKLPHRVDGTGFVVYDGALFFNKERTRNIVKFD
LRTRIKSGEAIIANANYHDTSPYRWGGKSDIDLAVDENGLWVIYATEQNNGKIVISQLNP
YTLRIEGTWDTAYDKRSASNAFMICGILYVVKSVYEDDDNEATGNKIDYIYNTDQSKDSL
VDVPFPNSYQYIAAVDYNPRDNLLYVWNNYHVVKYSLDFGPLDSRSGQAHHGQVSYISPP
IHLDSDLERPPVREISTTGPLGTGSTTTSTTLRTTTWSPGRSTTPSVSGRRN*532RSTSTPSP
AIEVLNDITTHVPSASPQIPALEESCEAVEAREIMWFKTRQGQMAKQPCPAGTIGVSTYL
CLAPDGIWDPQGPDLSN*617CSSPWVNHITQKLKSGETAANIARELAEQTRNHLNAGDITYSV
RAMDQLVGLLDVQLRNLTPGGKDSAARSLNKLQKRERSCRAYVQAMVETVNNLLQPQALN
AWRDLTTSDQLRAATMLLDTVEESAFVLADNLLKTDIVRENTDNIQLEVARLSTEGNLED
LKFPENTGHGSTIQLSANTLKQNGRNGEIRVAFVLYNNLGPYLSTEN*827ASMKLGTEAMSTN*840
HSVIVNSPVITAAINKEFSNKVYLADPVVFTVKHIKQSEENFNPN*885CSFWSYSKRTMTGYW
STQGCRLLTTN*911KTHTTCSCNHLTNFAVLMAHVEVKHSDAVHDLLLDVITWVGILLSLVCL
LICIFTFCFFRGLQSDRNTIHKNLCISLFVAELLFLIGIN*1000RTDQPIACAVFAALLHFFFL
AAFTWMFLEGVQLYIMLVEVFESEHSRRKYFYLVGYGMPALIVAVSAAVDYRSYGTDKVC
WLRLDTYFIWSFIGPATLIIMLNVIFLGIALYKMFHHTAILKPESGCLDNINYEDNRPFI
KSWVIGAIALLCLLGLTWAFGLMYIN*1166ESTVIMAYLFTIFNSLQGMFIFIFHCVLQKKVRK
EYGKCLRTHCCSGRSTESSIGSGKTSGSRTPGRYSTGSQSRIRRMWNDTVRKQS*1254ESSFIT
GDINSSASLNRGAMANHLISNALLRPHGTNNPYNTLLGEPAVCNNPSVSMYNAQEGLLNN
ARDTSVMDTLPLNGNHGNSYSIASGEYLSNCVQIIDRGYNHNETALEKKILKELTSNYIP
SYLNNHERSSEQNRNLMNKLVNNLGSGSEDDAIVLDDATSFNHEESLGLELIHEESDAPL
LPPRVYSTENHQLHHYTRRRIPQDHSESFFPLLTNEHTEDLQSPHRDSLYTSMPALAGVP
TAESVTTSTQTEPPPAKCGDAEDVYYKSMPNLGSRNHVHQLHTYYQLGRGSSDGFIVPPN
KDGTPPEGSSKGPAHLVTSL
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| Predicted Disorder Regions | 523-527, 1222-1235, 1400-1420, 1464-1469, 1497-1522, 1564-1568 |
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| DisProt Annotation | |
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| TM Helix Prediction | 6TMHs; (948-970), (1006-1028), (1051-1069), (1089-1111), (1143-1165), (1172-1194) |
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| Significance of PTMs | Proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. |
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| Linking IDs | |
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| Bibliography | Mathiasen S, Palmisano T, Perry NA, Stoveken HM, Vizurraga A, McEwen DP, Okashah N, Langenhan T, Inoue A, Lambert NA, Tall GG, Javitch JA. G12/13 is activated by acute tethered agonist exposure in the adhesion GPCR ADGRL3. Nat Chem Biol. 2020 Dec;16(12):1343-1350. doi: 10.1038/s41589-020-0617-7. Epub 2020 Aug 10. Erratum in: Nat Chem Biol. 2020 Aug 17;: PMID: 32778842; PMCID: PMC7990041. |
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