Primary Information |
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| BoMiProt ID | Bomi9955 |
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| Protein Name | Transcriptional repressor NF-X1/Nuclear transcription factor, X box-binding protein 1 |
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| Organism | Bos taurus |
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| Uniprot ID | A6QLA0 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001095499.1 |
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| Aminoacid Length | 1116 |
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| Molecular Weight | 123581 |
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| FASTA Sequence |
Download |
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| Gene Name | NFX1 |
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| Gene ID | 515680 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | nuclear proteins with the ability to bind to X-box cis-elements.bound a conserved X-box motif of MHC class II genes and transcriptionally repressed gene expression. its two splice variants protein products (NFX1-91 and NFX1-123) that are expressed in epithelial cells.bind to DNA (NFX1-91) and RNA (NFX1-123), and they are involved in gene expression regulation through transcriptional repression, transcriptional activation, and post-transcriptional mRNA stabilization. |
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| Biochemical Properties | NFX1-91 and NFX1-123 are two splice variants.They share a common N-terminus and central domain, but they have unique C-termini.In the N-terminus, there is a PAM2 motif. This motif is necessary for proteins to bind to cytoplasmic poly(A) binding proteins. In the central domain, there is a PHD/RING domain that has an E3 ubiquitin ligase function. There are also six cysteine-rich zinc-like fingers, which are required for DNA binding and are conserved from the murine homolog.For NFX1-91, its C-terminus is short and is lysine-rich. For NFX1-123, its unique C-terminus includes two additional zinc-like fingers and an R3H domain. |
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| PTMs | Phosphorylation on Ser |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A6QLA0|NFX1_BOVIN Transcriptional repressor NF-X1 OS=Bos taurus OX=9913 GN=NFX1 PE=2 SV=1
MAEAPPVSGAFKFNTDAAEFIPQERKNSGLYCGSQRRLDSNRIGRRNYSS*50PPPCHLSRQI
SYDDISAVHQHSFYPSGSKPKS*82QQTSFQSSVTNKS*95LKNHGLQNQPWQKLRSEKQHIRVKR
AQGLIDQTS*129DAPGLENVAKSESGTNLREHS*150PSESEKEIVGADPRGAKPKKATQFIYSYGR
GPKVKGKLKSEWGHRMTPKPEEAGPENTKPAGVIHPDSSDASSRKVVADGARRGEQRRHP
QKRSPWEVEGARPRPGRNPPKQEGQRHTNAGSRDNMASIPKDDLNERPAKSTCDSGNLAV
VSRSSRRADPEKCAVRRQDPQVAS*324FPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCE
LVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFC
GKVKNPEWSRNEIPHSCGEVCRRKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTR
HTVRCGQAVSVHCSNPCDNILNCGQHHCAELCHGGPCQPCRVILNQVCYCGSTSRDVLCG
TDIGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLSQ
LLELGSSGRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRC
SFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGL
HRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVY
HSCHSEDKCPPCTFLTQKWCMGKHELRSNIPCHLVDISCGLPCGATLPCGMHKCQRLCHK
GECLGDEICKQPCTTSRANCGHPCMAPCHLSLPCPVTTCKAKIELQCECGRRKEIMVCSE
ASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECSALERKKRLAEA
FHISDDSDPFNVRSSGSKFSDSLKEDARKDLRFVSDIEKEMEALVEAVNKGKSSKKSHCF
PPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAVRGKSICPSTTLTGVLEKENQSR
PPPPIAHHRQTDKNPGSSNLQKIAKEPVIDYFDVQD
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | NFX1-123 expression in keratinocytes mediated augmented activation of epithelial differentiation indirectly through Notch1. |
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| Bibliography | Chintala S, Katzenellenbogen RA. NFX1, Its Isoforms and Roles in Biology, Disease and Cancer. Biology (Basel). 2021 Mar 30;10(4):279. doi: 10.3390/biology10040279. PMID: 33808060; PMCID: PMC8067315. |
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