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Primary Information | |
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| BoMiProt ID | Bomi9811 |
| Protein Name | Thioredoxin-dependent peroxide reductase, mitochondrial/Antioxidant protein 1/Thioredoxin-dependent peroxiredoxin 3/Protein SP-22 |
| Organism | Bos taurus |
| Uniprot ID | P35705 |
| Milk Fraction | Whey |
| Ref Sequence ID | NP_776857.1 |
| Aminoacid Length | 257 |
| Molecular Weight | 28195 |
| FASTA Sequence | Download |
| Gene Name | PRDX3 |
| Gene ID | 281998 |
| Protein Existence Status | Reviewed |
Secondary Information | |
| Presence in other biological fluids/tissue/cells | liver, renal cortex and medulla,adrenal cortex and medulla |
| Protein Function | Prx3 plays an essential role in maintaining adipose tissue integrity and sustaining whole body metabolism. |
| PTMs | N6-Acetylation at Lys, Disulfide bond formation, Lipoylation, Palmitoylation, Phosphorylation at Thr |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|P35705|PRDX3_BOVIN Thioredoxin-dependent peroxide reductase, mitochondrial OS=Bos taurus OX=9913 GN=PRDX3 PE=1 SV=2 MAATAGRLFRASLIRHVSAIPWGISASAALRPAASRRMCLTNALWSGSDQAKFAFSTSSS YHAPAVTQHAPYFKGTAVVSGEFKEISLDDFKGKYLVLFFYPLDFTFVCPTEIIAFSDKA SEFHDVNCEVVAVSVDSHFSHLAWINT*147PRKNGGLGHMNIALLSDLTKQISRDYGVLLEGP GLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQFVEAHGEVCPANWTPESPT IKPHPTASREYFEKVNQ |
| SCOP | Class : Alpha and beta proteins (a/b) Fold : Thioredoxin fold Superfamily : Thioredoxin-like Family : Glutathione peroxidase-like Domain Name : 1ZYE A:2-163 |
| CATH | Matched CATH superfamily 3.40.30.10 |
| Predicted Disorder Regions | 234-257 |
| DisProt Annotation | |
| TM Helix Prediction | No TM helices |
| Significance of PTMs | Phosphorylation reduces perodixase activity.The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond. |
| PDB ID | 1ZYE, 4MH2, 4MH3, |
| Additional Comments | Transient overexpression of PRDX3 suppresses the sensitivity of FA-G cells to H2O2, and decreased PRDX3 expression increases sensitivity to mitomycin C. |
| Bibliography | 1.Watabe S, Kohno H, Kouyama H, Hiroi T, Yago N, Nakazawa T. Purification and characterization of a substrate protein for mitochondrial ATP-dependent protease in bovine adrenal cortex. J Biochem. 1994 Apr;115(4):648-54. doi: 10.1093/oxfordjournals.jbchem.a124390. PMID: 8089078. 2.Mukhopadhyay SS, Leung KS, Hicks MJ, Hastings PJ, Youssoufian H, Plon SE. Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia. J Cell Biol. 2006 Oct 23;175(2):225-35. doi: 10.1083/jcb.200607061. PMID: 17060495; PMCID: PMC2064564. 3.Huh JY, Kim Y, Jeong J, Park J, Kim I, Huh KH, Kim YS, Woo HA, Rhee SG, Lee KJ, Ha H. Peroxiredoxin 3 is a key molecule regulating adipocyte oxidative stress, mitochondrial biogenesis, and adipokine expression. Antioxid Redox Signal. 2012 Feb 1;16(3):229-43. doi: 10.1089/ars.2010.3766. Epub 2011 Oct 17. PMID: 21902452; PMCID: PMC3234662. 4. |