Primary Information
BoMiProt ID	Bomi9769
Protein Name	Terminal nucleotidyltransferase 5B
Organism	Bos taurus
Uniprot ID	Q29RH2
Milk Fraction	Whey
Ref Sequence ID	NP_001039415.1
Aminoacid Length	430
Molecular Weight	47097
FASTA Sequence	Download
Gene Name	TENT5B/FAM46B
Gene ID	506745
Protein Existence Status	reviewed
Secondary Information
Protein Function	These are newly identified metazoan-specific poly(A) polymerases (PAPs),selectively add a string of nontemplated AMPs to the 3′-end of target mRNAs by consuming ATP molecules.
Biochemical Properties	belong to nucleotidyltransferase (NTase) superfamily, a prominently larger N-terminal catalytic domain (NCD)as compared to known eukaryotic PAPs, and a C-terminal helical domain(HD).Asp91, Asp93 and Glu167 are present at the putative catalytic site.The NCD is built of a central eight-stranded β-sheet (β1−β8) surrounded by seven α-helices (α1−α7) involving a typical NTase core.The HD is composed of 5 α-helices which are associated with hydrophobic interactions.
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	na
Predicted Disorder Regions	(1-47)
DisProt Annotation
TM Helix Prediction	no TM helices
Additional Comments	Knock-down of FAM46B induces apoptosis and restricts protein synthesis. FAM46B inhibits cell proliferation and cell cycle progression in prostate cancer through ubiquitination of β-catenin.
Bibliography	1.Hu JL, Liang H, Zhang H, Yang MZ, Sun W, Zhang P, Luo L, Feng JX, Bai H, Liu F, Zhang T, Yang JY, Gao Q, Long Y, Ma XY, Chen Y, Zhong Q, Yu B, Liao S, Wang Y, Zhao Y, Zeng MS, Cao N, Wang J, Chen W, Yang HT, Gao S. FAM46B is a prokaryotic-like cytoplasmic poly(A) polymerase essential in human embryonic stem cells. Nucleic Acids Res. 2020 Mar 18;48(5):2733-2748. doi: 10.1093/nar/gkaa049. PMID: 32009146; PMCID: PMC7049688. 2.Liang T, Ye X, Liu Y, Qiu X, Li Z, Tian B, Yan D. FAM46B inhibits cell proliferation and cell cycle progression in prostate cancer through ubiquitination of β-catenin. Exp Mol Med. 2018 Dec 10;50(12):1-12. doi: 10.1038/s12276-018-0184-0. PMID: 30532005; PMCID: PMC6288130.