Primary Information
BoMiProt ID	Bomi9647
Protein Name	Synaptotagmin-1/Synaptotagmin I/p65
Organism	Bos taurus
Uniprot ID	P48018
Milk Fraction	Exosomes
Ref Sequence ID	NP_776617.1
Aminoacid Length	422
Molecular Weight	47623
FASTA Sequence	Download
Gene Name	SYT1
Gene ID	281511
Protein Existence Status	Reviewed
Secondary Information
Presence in other biological fluids/tissue/cells	occipital lobe
Protein Function	Synaptotagmin-1 (Syt1), a Ca2+ sensor, mediates ultrafast exocytosis in neurons and neuroendocrine cells.
Biochemical Properties	C2 domains of Syt1 have membrane-binding affinity and suggested Syt1 as the Ca2+ sensor.Acidic aspartate residues in two Ca2+-binding C2 domains (C2A and C2B), coordinate three and two Ca2+ ions, respectively. In addition to these Ca2+-binding loops, the C2B domain contains a polybasic region (KKKK, 324–327) that is close to Ca2+-binding loops and is enriched with lysine residues that interact with anionic phospholipids or the SNARE complex.
PTMs	N-Linked Glycosylation at Asn, Lipoylation, Palmitoylation, Phosphorylation at Ser/Thr
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|P48018|SYT1_BOVIN Synaptotagmin-1 OS=Bos taurus OX=9913 GN=SYT1 PE=1 SV=1 MVSESHHEALAAPPVTTVATVLPHN*25ATEPASPGEGKEDAFSKLKEKFMNELHKIPLPPWA LIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALK DDDAETGLT*129DGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDP YVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVY*230DFDRFSKHDI IGEFKVPMNTVDFGHVTEEWRDLQS*265AEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNL KKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNES*343FS*345FEVPFEQIQKVQVVV TVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAV KK
Predicted Disorder Regions	10-36, 84-140, 321-329
DisProt Annotation
TM Helix Prediction	1TMH; (58-80)
Additional Comments	Syt-1 is involved in the development of neuropathic pain and that EA attenuates neuropathic pain, probably through suppressing Syt-1 protein expression in the spinal cord.
Bibliography	1.Park Y, Ryu JK. Models of synaptotagmin-1 to trigger Ca2+ -dependent vesicle fusion. FEBS Lett. 2018 Nov;592(21):3480-3492. doi: 10.1002/1873-3468.13193. Epub 2018 Jul 30. PMID: 30004579. 2.Perin MS, Fried VA, Mignery GA, Jahn R and Sudhof TC (1990) Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C. Nature 345, 260– 263. 3.Fernandez I, Arac D, Ubach J, Gerber SH, Shin O, Gao Y, Anderson RG, Sudhof TC and Rizo J (2001) Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine. Neuron 32, 1057– 1069. 4.Sutton RB, Davletov BA, Berghuis AM, Sudhof TC and Sprang SR (1995) Structure of the first C2 domain of synaptotagmin I: a novel Ca2 + /phospholipid-binding fold. Cell 80, 929– 938. 5.Ubach J, Zhang X, Shao X, Sudhof TC and Rizo J (1998) Ca2 + binding to synaptotagmin: how many Ca2 + ions bind to the tip of a C2-domain? EMBO J 17, 3921– 3930. 6.Arac D, Chen X, Khant HA, Ubach J, Ludtke SJ, Kikkawa M, Johnson AE, Chiu W, Sudhof TC and Rizo J (2006) Close membrane-membrane proximity induced by Ca(2 + )-dependent multivalent binding of synaptotagmin-1 to phospholipids. Nat Struct Mol Biol 13, 209– 217.