Primary Information
BoMiProt ID	Bomi9618
Protein Name	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1/60 kDa BRG-1/Brm-associated factor subunit A/BRG1-associated factor 60A/ BAF60A/SWI/SNF complex 60 kDa subunit
Organism	Bos taurus
Uniprot ID	Q2TBN1
Milk Fraction	whey
Ref Sequence ID	NP_001033648.1
Aminoacid Length	515
Molecular Weight	58252
FASTA Sequence	Download
Gene Name	SMARCD1/BAF60A
Gene ID	533232
Protein Existence Status	Reviewed
Secondary Information
Protein Function	Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner
Biochemical Properties	SMARCAD1 contains two N-terminal CUE domains (CUE1, CUE2), which are equivalent to UBD.
PTMs	Acetylation, methylation, phosphorylation,ubl conjugation
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|Q2TBN1|SMRD1_BOVIN SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 OS=Bos taurus OX=9913 GN=SMARCD1 PE=2 SV=1 MAARAGFQSVAPSGGAGASGGAGAAAALGPGGTPGPPVRMGPAPGQGLYRSPMPGAAYPR PGMLPGSRMTPQGPSMGPPGYGGNPSVRPGLAQSGMDQSLKRPAPQQIKQVQQQAVQNRN HNAKKKKMADKILPQRIRELVPESQDYMDLLAFERKLDQTIMRKRLDIQEALKRPIKQKR KLRIFISNTFNPAKSDAEDGEGT*203VASWELRVEGRLLEDSALSKYDATKQKRKFSSFFKSL VIELDKDLYGPDNHLVEWHRTATTQETDGFQVKRPGDVNVRCTVLLMLDYQPPQFKLDPR LARLLGIHTQTRPVIIQALWQYIKTHKLQDPHEREFVICDKYLQQIFESQRMKFSEIPQR LHALLMPPEPIIINHVISVDPNDQKKTACYDIDEEVDDTLKTQMNSFLLSTASQQEIATL DNKIHETIETINQLKTQREFMLSFARDPQGFINDWLQSQCRDLKVMTDVVGNSEEERRAE FYFQPWAQEAVCRYFYSKVQQRRQELEQALGIRNT
Predicted Disorder Regions	1-131, 194-201
DisProt Annotation
TM Helix Prediction	No TM helices
Significance of PTMs	Ionizing radiation (IR) induces SMARCAD1 phosphorylation at a conserved T906 by ATM kinase, a modification essential for SMARCAD1 recruitment to DSBs.T906 phosphorylation is also important for SMARCAD1 ubiquitination by RING1 at K905.
Additional Comments	SMARCAD1 facilitates the recruitment of BRCA1 at the DNA break site and that SMARCAD1 phosphorylation at T906 and its ATPase activity are required for BRCA1 localization at break sites.
Bibliography	1.Chakraborty, S., Pandita, R. K., Hambarde, S., Mattoo, A. R., Charaka, V., Ahmed, K. M., Iyer, S. P., Hunt, C. R., & Pandita, T. K. (2018). SMARCAD1 Phosphorylation and Ubiquitination Are Required for Resection during DNA Double-Strand Break Repair. iScience, 2, 123–135. https://doi.org/10.1016/j.isci.2018.03.016 2.van de Wijngaart, D. J., Dubbink, H. J., Molier, M., de Vos, C., Trapman, J., & Jenster, G. (2009). Functional screening of FxxLF-like peptide motifs identifies SMARCD1/BAF60a as an androgen receptor cofactor that modulates TMPRSS2 expression. Molecular endocrinology (Baltimore, Md.), 23(11), 1776–1786. https://doi.org/10.1210/me.2008-0280