Primary Information
BoMiProt ID	Bomi9550
Protein Name	Stromal membrane-associated protein 2/Stromal membrane-associated protein 1-like
Organism	Bos taurus
Uniprot ID	Q5EA00
Milk Fraction	whey
Ref Sequence ID	NP_001069138.1
Aminoacid Length	429
Molecular Weight	46900
FASTA Sequence	Download
Gene Name	SMAP2/SMAP1L
Protein Existence Status	Reviewed
Secondary Information
Protein Function	Stromal membrane-associated protein is basically GTPase activating protein that acts on ARF1. It can also activate ARF6. Additionally, it may also be involved in clathrin-dependent retrograde transport from early endosome to trans-Golgi network (TGN).
Biochemical Properties	The polypeptide encoded by SMAP2 is composed of 428 aa residues and has a predicted molecular mass of 47 kDa.SMAP2 possesses a GAP domain and interacts with clathrin heavy chain (CHC).The amino-terminal region (aa 16-125) of SMAP2 harbors a conserved CX2CX16CX2C zinc finger motif and is strongly homologous to the corresponding region of SMAP1. Therefore, it is possible that SMAP2, like SMAP1, exhibits ArfGAP activity.The second conserved region of SMAP2 is contained by aa 173-212. This region harbors both classical and atypical CHC(clathrin)-binding motifs such as LLGLD (aa 187-191) and DLL (aa 212-214), respectively.LLGLD motif in SMAP1 functions as a CHC-binding motif
PTMs	Phosphorylation
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|Q5EA00|SMAP2_BOVIN Stromal membrane-associated protein 2 OS=Bos taurus OX=9913 GN=SMAP2 PE=2 SV=1 MTGKSVKDVDRYQAVLANLLLEEDNKFCADCQSKGPRWASWNIGVFICIRCAGIHRNLGV HISRVKSVNLDQWTQEQIQCMQEMGNGKANRLYEAYLPETFRRPQIDHAVEGFIRDKYEK KKYMDRS*127LDINAFRKEKDNKWKRGSEPAPEKKMEPVVFEKVKMPQKKEDPQLPRKTSPKS KAPVVDLLGLDAPVSCSIANGKTSNTLEKDLDLLASVSS*219PSSS*223VS*225RKVVGS*231MPTPGSAGS*240VPENLNLFPEPGSKSEETSKKQLSKDSILSLYGSQTPQMPTQAMFMAPAQMAYPTAYPSF PGVTPPNSLMGSMMPPPVGMVAQPGASGMVAPMAMPAGYMGGMQASMMGVPNGMMTTQQA SYMAGMAAMPQTMYGVQPAQQLQWNLTQMTQQMAGMNFCGTNGMLSYGQSMNGGNGQAAN QTLSPQMWK
Predicted Disorder Regions	132-274, 287-326, 399-401, 410-429
DisProt Annotation
TM Helix Prediction	No TM helices
Bibliography	1.Natsume W, Tanabe K, Kon S, Yoshida N, Watanabe T, Torii T, Satake M. SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network. Mol Biol Cell. 2006 Jun;17(6):2592-603. doi: 10.1091/mbc.e05-10-0909. Epub 2006 Mar 29. PMID: 16571680; PMCID: PMC1475504. 2.Harhay, G. P., Sonstegard, T. S., Keele, J. W., Heaton, M. P., Clawson, M. L., Snelling, W. M., Wiedmann, R. T., Van Tassell, C. P., & Smith, T. P. (2005). Characterization of 954 bovine full-CDS cDNA sequences. BMC genomics, 6, 166. https://doi.org/10.1186/1471-2164-6-166