Primary Information |
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| BoMiProt ID | Bomi9527 |
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| Protein Name | Sterol regulatory element-binding protein cleavage-activating protein/SREBP cleavage-activating protein/SCAP |
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| Organism | Bos taurus |
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| Uniprot ID | A6QM06 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001095359.1 |
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| Aminoacid Length | 1278 |
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| Molecular Weight | 139701 |
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| FASTA Sequence |
Download |
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| Gene Name | SCAP |
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| Gene ID | 507878 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Regulation of cholesterol homeostasis.An important role of SCAP-SREBP2 that is directly used by NLRP3 for inflammasome activation, thus suggesting an intimate metabolic-inflammatory
crosstalk in pro-inflammatory macrophages. |
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| Biochemical Properties | In addition to controlling cholesterol biosynthesis, SCAP-SREBP2 also serves as a signaling hub integrating cholesterol metabolism with inflammation in macrophages. |
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| Significance in milk | A ubiquitous expression profile of SCAP gene was detected in various tissues with extreme predominance in the mammary gland during early lactation.Buffalo SCAP could sever as a candidate gene affecting milk production traits in buffalo and the identified SNPs might potentially be genetic markers. |
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| PTMs | N-Linked Glycosylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A6QM06|SCAP_BOVIN Sterol regulatory element-binding protein cleavage-activating protein OS=Bos taurus OX=9913 GN=SCAP PE=2 SV=1
MTLTERLREKISQAFYNHGLFCASYPIPIILFTGLCILACCYPLLKLPLPGTGPVEFTTP
VKDYSPPPLTSDHKPGEPNEQPEWYVGAPVAYIQQIFVKSSVSPWHKNLLAVDVFRSPLS
RAFQLVEEIRNHVLKDSSGTRSLEDVCLQVTDLLPGLRKLRNLLPEHGCLLLSPGNFWQN
DRERFHADPDIIRTIHQHEPKTLQTSATLKDLLFGVPGKYSGVSLYTRKRLVSYTITLVF
QHYHAKFLGSLRARLMLLHPSPN*263CSLRAESLVHVHFKEEIGIAELIPLVTTYIILFAYIY
FSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLCTLFGLTPTLNGGEIFPYLVVVIGLEN
VLVLTKSVVSTPVDLEVKLRIAQGLSSESWSIMKNMATELGIVLIGYFTLVPAIQEFCLF
AVVGLVSDFFLQMLFFTTVLSIDIRRMELADLNKRLPPEACLPPAKPVGRPTRFERQPTV
RPSMPHTITLQPSSFRNLRLPKRLRVIYFLARTRLAQRLIMAGTVVWIGILVYTDPAGLR
TYLAAQVTEQSPLGEGALAPLPVPSGVLPASHPDPAFSIFPPDASKLPEN*590QTLPGEPPEP
GGLAEGVHDSPAPEVTWGPEDEELWRKLSFRHWPTLFSYYN*641ITLAKRYVSLLPVIPVTLR
LNPREALEGRHPQDGRSAWPPPRPGQGGLWEAGPKGPGTAQAQRDLTLYKVAALGLASGI
VLVLLLLCLYRVLCPRNYGQPGAGPGRRRRGELPCDDYGYAPPETEIVPLVLRGHLMDIE
CLASDGMLLVSCCLAGHVCVWDAQTGDCLTRIPHPGQRRDS*821GVGSGLETQETWERLS*837DGG
KGGPEEPGDS*850PPLRHRPRGPPPPALFGDQPDLTCLIDTNFSARPQLPEPAQPEPRYRAGR
RAQDS*905AGYDFSRLVQRVYQEGGMAPVHTPALRPPS*935PGPTFPLAPEDEAGFPPEKSCPSLA
WAPSTDGSIWSLELQGSLIVVGRSSGRLEVWDAIEGTLRCSSEEVSSGITALVFLDRRIV
AARLNGSLDFFSLETHTALSPLQFRGAPGRGSSPASPACSSSDRVACHLTHTVPCAHQKP
ITALKAAAGRLVTGSQDHTLRVFRLEDSCCLFTLQGHSGAITTVYIDQTMVLASGGQDGA
ICLWDVLTGSRVSHMFAHRGDVTSLTCTTSCVISSGLDDLISIWDRSTGIKLYSIQQDLG
CGASLGVISDNLLVTGGQGCVSFWDLNYGDLLQTVYLGKNSEAQPARQILVLDNAAIVCN
FGSELSLVYVPSVLEKLD
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| Predicted Disorder Regions | 65-72, 836-846, 880-893, 934-951 |
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| DisProt Annotation | |
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| TM Helix Prediction | 7TMHs; (20-42), (280-302), (315-333), (348-370), (403-425), (515-533), (711-733) |
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| Significance of PTMs | N-glycosylation of SCAP Is Essential for SREBP-1 Activation and Tumor Growth |
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| Additional Comments | NLRP3 associated with SCAP-SREBP2 to form a ternary complex which translocated to the Golgi apparatus adjacent to a mitochondrial cluster for optimal inflammasome assembly. |
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| Bibliography | 1.Guo C, Chi Z, Jiang D, Xu T, Yu W, Wang Z, Chen S, Zhang L, Liu Q, Guo X, Zhang X, Li W, Lu L, Wu Y, Song BL, Wang D. Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. Immunity. 2018 Nov 20;49(5):842-856.e7. doi: 10.1016/j.immuni.2018.08.021. Epub 2018 Oct 23. PMID: 30366764. 2.Cheng C, Ru P, Geng F, Liu J, Yoo JY, Wu X, Cheng X, Euthine V, Hu P, Guo JY, Lefai E, Kaur B, Nohturfft A, Ma J, Chakravarti A, Guo D. Glucose-Mediated N-glycosylation of SCAP Is Essential for SREBP-1 Activation and Tumor Growth. Cancer Cell. 2015 Nov 9;28(5):569-581. doi: 10.1016/j.ccell.2015.09.021. PMID: 26555173; PMCID: PMC4643405. 2.Deng T, Ma X, Pang C, Liang S, Lu X, Duan A, Liang X. Molecular characterisation of the buffalo SCAP gene and its association with milk production traits in water buffaloes. J Dairy Res. 2018 May;85(2):133-137. doi: 10.1017/S0022029918000195. PMID: 29785906. |
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