Primary Information |
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| BoMiProt ID | Bomi9459 |
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| Protein Name | Spindle and centriole-associated protein 1/Coiled-coil domain-containing protein 52 |
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| Organism | Bos taurus |
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| Uniprot ID | Q2T9X8 |
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| Milk Fraction | Whey |
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| Aminoacid Length | 860 |
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| Molecular Weight | 95713 |
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| FASTA Sequence |
Download |
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| Gene Name | SPICE1/CCDC52 |
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| Gene ID | 515350 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Presence in other biological fluids/tissue/cells | spindle microtubules |
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| Protein Function | required for centriole duplication and for proper bipolar spindle formation and chromosome congression in mitosis.Centriole elongation |
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| Biochemical Properties | well conserved two potential coiled-coil regions (CC1 and CC2). central conserved CC1 region is involved in centriole localization. |
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| PTMs | phosphorylation on Ser and Thr |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q2T9X8|SPICE_BOVIN Spindle and centriole-associated protein 1 OS=Bos taurus OX=9913 GN=SPICE1 PE=2 SV=1
MSFVRVNRYGPRGGGRKTLKVKKKTSVKQEWDNTVTDLTVHRATPEDLIRRHEIHKSKNR
ALVHWELQEKALKRRWKKQKPEISNLEKRRLSIMKEILSDQYQLQDVLEKSDHLMATAKG
LFVDFPRRRTGFPNVTMAPESSQSPTVVSKDPVTQAIRSESVIEPQALNEVDDDEQEGTV
NSQSEESENELDNSLSSQSNANAGTFLHQIKEENSELINKLWTDIQQKVATQSQMT*236ASSG
TPSSASPSGEQKAALNATNAVKRIHTRLQPEESTETLDSSYVVGQVLNSRKQKQLLNKVK
RKPDSRAPSKQKSSMLSASTASTDLPSSSNPSLDVLKHMIHEVEHEIEEYERWTGREVQG
LQNSQGLTGFTLSLVSSLCRLVRYLKESELQLRKEVETRQRLEEALGDHRELIDALTAEV
LFLREENTATQARLQQYMITTDEQLISLTHAIKNCPVISNKESQALERGATSQRHIDNPE
DPAVNASVSMPLMFRGEDVVEFPQEDLPVKLSQVPNPPESGDLASSLPGHIFEPAVLLTP
PRQKSNSEFSPLQDVLRRTVQTRPAPRIPPTVEIIEKEQNWEKKTLPVGADIQNSSDESH
LFTQRWRASHMGEDSQSKTQAPFVSLSQPLCSSQSSMQQSRNPTLS*646EEPLVLGDGQQLRT
NETLIQRKDIMARIAELTLQNSDIRAHLNNIIGPGGEQGDGLREFNRQETSHASDTMATF
PAVQPLTPSSMEERIAELNRQSMEARGKLLQLIEQQKLLGLNPSS*765PPVS*769PVQSPLRAWAE
GGKRTIEVSIPGAEAPESSKCSTDSPTSGLNSRRSSGAASNSCS*824PLNATSGSGRLTPLNP
RAKIEKQNEEGWFALSTHVS |
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| Predicted Disorder Regions | 13-26, 154-222, 242-258, 295-328, 613-653, 711-718, 797-831 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | Constitutive phosphorylation of SPICE1 c terminal region by Aurora Kinase results in abnormal centriole number, chromosome alignment defects and spindle multipolarity.In the absence of Aurora kinase activity, SPICE1 remains at centrioles but does not target to the spindle.549–855 residues of SPICE1, as a novel substrate of Aurora kinases in cells. |
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| Additional Comments | Depletion of SPICE causes defects in the mitotic spindle and impairs chromosome congression |
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| Bibliography | 1.Archinti M, Lacasa C, Teixidó-Travesa N, Lüders J. SPICE--a previously uncharacterized protein required for centriole duplication and mitotic chromosome congression. J Cell Sci. 2010 Sep 15;123(Pt 18):3039-46. doi: 10.1242/jcs.069963. Epub 2010 Aug 24. PMID: 20736305.2.Comartin D, Gupta GD, Fussner E, Coyaud É, Hasegan M, Archinti M, Cheung SW, Pinchev D, Lawo S, Raught B, Bazett-Jones DP, Lüders J, Pelletier L. CEP120 and SPICE1 cooperate with CPAP in centriole elongation. Curr Biol. 2013 Jul 22;23(14):1360-6. doi: 10.1016/j.cub.2013.06.002. Epub 2013 Jun 27. PMID: 23810536. 3.Deretic J, Kerr A, Welburn JPI. A rapid computational approach identifies SPICE1 as an Aurora kinase substrate. Mol Biol Cell. 2019 Feb 1;30(3):312-323. doi: 10.1091/mbc.E18-08-0495. Epub 2018 Nov 28. PMID: 30485161; PMCID: PMC6589576. |
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