Primary Information |
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| BoMiProt ID | Bomi9057 |
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| Protein Name | Serine/threonine-protein kinase VRK1/Vaccinia-related kinase 1 |
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| Organism | Bos taurus |
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| Uniprot ID | Q32PI1 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001033313.1 |
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| Aminoacid Length | 396 |
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| Molecular Weight | 45358 |
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| FASTA Sequence |
Download |
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| Gene Name | VRK1 |
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| Gene ID | 618880 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | VRK1 regulates entry in cell cycle, chromatin condensation in G2/M, Golgi fragmentation, Cajal body dynamics and nuclear envelope assembly in mitosis. This kinase also controls the initial chromatin relaxation associated with histone acetylation, and the non-homologous-end joining (NHEJ) DNA repair pathway. |
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| Biochemical Properties | VRK1 has a strong autophosphorylating activity in several Ser and Thr residues.VRK-1 phosphorylates acidic proteins, such as phosvitin and casein, and basic proteins such as histone 2b and myelin basic protein. |
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| PTMs | Isopeptide bond, Phosphorylation at Ser, Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| NA |
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| Predicted Disorder Regions | 1-20, 331-336, 344-396 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | Depletion of the protein kinase VRK1 disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes |
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| Bibliography | 1.Lopez-Borges S, Lazo PA. The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein. Oncogene. 2000 Jul 27;19(32):3656-64. doi: 10.1038/sj.onc.1203709. PMID: 10951572. 2.Campillo-Marcos I, García-González R, Navarro-Carrasco E, Lazo PA. The human VRK1 chromatin kinase in cancer biology. Cancer Lett. 2021 Apr 10;503:117-128. doi: 10.1016/j.canlet.2020.12.032. Epub 2021 Jan 29. PMID: 33516791. 3.Molitor TP, Traktman P. Depletion of the protein kinase VRK1 disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes. Mol Biol Cell. 2014 Mar;25(6):891-903. doi: 10.1091/mbc.E13-10-0603. Epub 2014 Jan 15. PMID: 24430874; PMCID: PMC3952857. |
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