Primary Information |
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| BoMiProt ID | Bomi9046 |
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| Protein Name | Serine/threonine-protein kinase greatwall/Microtubule-associated serine/threonine-protein kinase-like/GW/GWL/MAST-L |
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| Organism | Bos taurus |
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| Uniprot ID | E1BFR5 |
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| Milk Fraction | Whey |
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| Aminoacid Length | 883 |
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| Molecular Weight | 98072 |
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| FASTA Sequence |
Download |
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| Gene Name | MASTL/GW/GWL |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | mitosis-accelerating kinase plays as an activator of cell contractility and MRTF-A/SRF (myocardin-related transcription factor A/serum response factor) signaling. |
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| Biochemical Properties | promotes normal G2-mitosis transition by inhibiting PP2A via ARPP19 and ENSA.Constitutively active MASTL promoted dephosphorylation of CDK1(Tyr15) and accelerated mitotic entry after DNA damage. |
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| PTMs | Phosphorylation on Ser and Thr,Acetylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|E1BFR5|GWL_BOVIN Serine/threonine-protein kinase greatwall OS=Bos taurus OX=9913 GN=MASTL PE=3 SV=1
MEPTMGGEMESGGGAATGECVNRIPVPRPPSIEEFTIVKPISRGAFGKVYLGQKGNRLYA
VKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKS
LLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKV
TLNRDIDINMMDILTTPSMAKPRQDYSRT*209PGQVLSLISSLGFHT*224PVAEGNHDTANVLSTQ
VSETSPLSQGLTCPMSVDQKDTTPYSSKLLKSCPEMVASHPRMPVKCLTSHLLQS*295RKRLA
TSSTSSPSHTFISSMESECHSSPRWEKDCQESDDAAGSTMMSWNTVEKPLCTKSVDAMET
KSFNERDLELALS*373PIHNSSVVPATGNSYVNLAKKCSSGEVSWEARELDVNNINMTADTSQ
YCFHESNQRAVDSGGMTEEHLGKRSCKRIFELVDSS*456PRQGIIPNKKSCFEYECSNEMRDC
YATQRTGFAFEVQDLKLLVYRDQQNDCVNKENVGSSFTDKHQT*523PEKSPVPMIEKNLMCEL
DDDCDKNSKKDYLSSS*556FLCS*560DGDRTPKSIHMDSDSSFPGISIMESPLGGQSLDPDKNIKE
SSLEESNIEDLLPVSPSCQESTLPKGVECPTIQDS*635NQKMLAPSSEVLKPLTSKRNAVAFR
S*661FNSHINASNSS*672EPSKMSITSLDMMDVSCAYSGSYPTAITPTQRERSDMPYQQTPNQVKS
ETPYRT*726PKS*729VRRGAAPVDDGRILGT*745PDYLAPELLLARAHGPAVDWWALGVCLFEFLTGIP
PFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVDILLTIDDTKRAGMKELKHHPLFS
GVDWENLQHQKMPFIPQPDDETDTSYFEARNNAQHLTVS*879GFS*882L |
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| Predicted Disorder Regions | 3-24, 332-337, 590-649, 667-674, 701-720 |
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| DisProt Annotation | |
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| TM Helix Prediction | no TM helices |
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| Significance of PTMs | Phosphorylated(Phosphorylation at Thr-745,Ser875,Thr194, Thr207, S213 and Thr741 by CDK1) and auto-phosphorylation on Ser875 during mitosis entry,which is essential for its activity.during mitosis exit again dephosphorylated by PP1 phosphatase.Deactivation of MASTL is essential for mitotic exit. |
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| Bibliography | 1.Rogers S, Fey D, McCloy RA, Parker BL, Mitchell NJ, Payne RJ, Daly RJ, James DE, Caldon CE, Watkins DN, Croucher DR, Burgess A. PP1 initiates the dephosphorylation of MASTL, triggering mitotic exit and bistability in human cells. J Cell Sci. 2016 Apr 1;129(7):1340-54. doi: 10.1242/jcs.179754. Epub 2016 Feb 12. PMID: 26872783; PMCID: PMC4852720. 2.Wong PY, Ma HT, Lee HJ, Poon RY. MASTL(Greatwall) regulates DNA damage responses by coordinating mitotic entry after checkpoint recovery and APC/C activation. Sci Rep. 2016 Feb 29;6:22230. doi: 10.1038/srep22230. PMID: 26923777; PMCID: PMC4770598. |
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