Primary Information
BoMiProt ID	Bomi8888
Protein Name	RNA polymerase II-associated protein 1
Organism	Bos taurus
Uniprot ID	A0JN53
Milk Fraction	Whey
Ref Sequence ID	NP_001071452.1
Aminoacid Length	1395
Molecular Weight	152859
FASTA Sequence	Download
Gene Name	RPAP1
Gene ID	531362
Protein Existence Status	reviewed
Secondary Information
Protein Function	The RNA polymerase II-associated protein 1 (RPAP1) is conserved across metazoa and required for stem cell differentiation in plants.RPAP1 is essential for the expression of cell identity genes and for cell viability. RPAP1 is essential for the interaction between RNA polymerase II (RNA Pol II) and Mediator, as well as for the recruitment of important regulators, such as the Mediator-specific RNA Pol II factor Gdown1 and the C-terminal domain (CTD) phosphatase RPAP2.
Biochemical Properties	RPAP1 is a large (153-kDa) multidomain protein with a high degree of conservation across species.Studies in plants, yeasts, and mammals indicate that RPAP1 interacts with the RPB3 (official name POLR2C) and RPB11 (POLR2J) subunits of the RNA Pol II complex. Importantly, the heterodimer RPB3/RPB11 provides a critical interface of RNA Pol II with the Mediator complex.
PTMs	Phosphorylation at Ser
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|A0JN53|RPAP1_BOVIN RNA polymerase II-associated protein 1 OS=Bos taurus OX=9913 GN=RPAP1 PE=2 SV=1 MLSRPKPGESEVDLLRFQSQFLAAGATPAVQLVKKGSRRAGDANLEQPPLQDHRDVVMLD SLPDLPPALVPAPPKRARPSPGHLLPEHEDPEERLHRHDQHITAVLTKIIERDTSSMPVN LPVSSGVAFPPVFHRSQGRQGKPVTAGKRSIFAQEIAARRASGAKVSPVREVESILDPPE SAMTCEALTPREWGSQPPWNSYSFQGPHLVTGKGLKGQEAEQEAQTIHEENVARLQALAP EEILQEQQRLLAQLDPSLVAFLKSHSCTREQAEEKATREQRPGRPSAEVIGKEAIAPTSA SVPRQENELEPETPALALPVT*321PQKEWLHMDTVELEKLHWTQDLPPLRRQQTQERMQARFS LQGELLAPDMDLPTHLGLHHHGEEAERAGYSLQELFHLTRSQVSQQRALALHVLAQVIGR AQAGEFGDRLVGSVLHLLLDAGFLFLLRFSLDDRVDGVIAAAVRALRALLVAPGDEELLD STFSWYHGALMFALMPSQEDKEDEDEDEEPPAEKAKTKSPEEGNRPPSDLARHDIIKGLL ATNLLPRLRYVLEVTCPGPSVVLDILTVLIRLARHSLESATRVLECPRLVETVVREFLPT SWSPMGSGPTSSLHRVPCAPAMKLLRVLASASRNIAARLLSGFDLRSRLSRFIAEDPQDL ALPLEEAETLSTEAFRLWAVAASYGLGSDLYRELYPVLMQALQDVPKELSSPPPRPLAVQ RIASLLTLLTQLTLAAGHIAPEHNSHSAEASLLAGSSSVTWTQVSGLQPLVEPCLRQTLK LLPRPEMWSALGPVPTACLLFLDAYYQAWSQQPGLCPEDWLQDMERLSEGLLLPLLKHPS LGSLWDSLGCCSPLCNPQSCAVAPETISSLASLGCAGGHPPLSLAGSASPFPFLTALLSL LNTLGRIHKGLCGQLATVLAAPGLQDYFLRCVAPVAALHLTPFSAWALRHEYHLQYLALT LAQRAATLQPPMSGTDAALCHGMALALLGRLLPGSEHLAHELMLSCVFRLEFLPERASGG PEAADFSDRLSLGSGGDLGCGRGALLAQACQDLPSIRSCYLTHCSLARASLLASQALYRG ELQRVPALLLPVPKEPLLPTDWPFLPLIQLYHRASDTPSGLPPADTVGTALRALQWVLVL ESWRPRALWAVSPAARLARLMCVFLVDSELFRETPIQRLVAALLARLCQPEVLSNLNLDC PLPGLTSFPDLYANFLEHFEAVSFGDHLFGALILLPLQRRFSVTLRLALFGEHVGALRAL GLPLTQLPVSLECYTAPPEDNLALLQLYFRALVTGALRPRWCPVLYAVAVAHVNSFIFSQ DPNNSDEIKAACRSMLQKTWLLTDEGLRQHLLHYKLPNSALPEGFELYPQLPPLRQQYLQ KLRISGVLPNGVSDT
Predicted Disorder Regions	NA
DisProt Annotation
TM Helix Prediction	No TM helices
Additional Comments	Depletion of RPAP1 triggers cell de-differentiation, facilitates reprogramming toward pluripotency, and impairs differentiation. Direct interactions with stomatal transcription factors provide a novel mechanism by which RPAP proteins may control differentiation of cell types and tissues in eukaryotes.
Bibliography	1.Lynch CJ, Bernad R, Calvo I, Nóbrega-Pereira S, Ruiz S, Ibarz N, Martinez-Val A, Graña-Castro O, Gómez-López G, Andrés-León E, Espinosa Angarica V, Del Sol A, Ortega S, Fernandez-Capetillo O, Rojo E, Munoz J, Serrano M. The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity. Cell Rep. 2018 Jan 9;22(2):396-410. doi: 10.1016/j.celrep.2017.12.062. PMID: 29320736; PMCID: PMC5775503. 2.Chen L, Zhao M, Wu Z, Chen S, Rojo E, Luo J, Li P, Zhao L, Chen Y, Deng J, Cheng B, He K, Gou X, Li J, Hou S. RNA polymerase II associated proteins regulate stomatal development through direct interaction with stomatal transcription factors in Arabidopsis thaliana. New Phytol. 2021 Apr;230(1):171-189. doi: 10.1111/nph.17004. Epub 2020 Nov 23. PMID: 33058210.