Primary Information |
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| BoMiProt ID | Bomi8737 |
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| Protein Name | Retinol dehydrogenase 8/Photoreceptor outer segment all-trans retinol dehydrogenase |
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| Organism | Bos taurus |
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| Uniprot ID | Q9N126 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_776592.1 |
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| Aminoacid Length | 312 |
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| Molecular Weight | 33956 |
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| FASTA Sequence |
Download |
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| Gene Name | RDH8/PRRDH |
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| Gene ID | 281449 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Retinol dehydrogenase 8 (RDH8) reduces all-trans retinal (ATRal) into all-trans retinol, which is the first step of the visual cycle. |
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| Biochemical Properties | Bovine RDH8 contains only two tryptophan residues, which are both located in its C-terminus (W289 and W310).These are short-chain dehydrogenases/reductases (SDR).Contains Rossmann fold.Membrane binding and targeting of RDH8 to the outer segment of photoreceptors is guided by its last 16 amino acids The amphipathic helical structure in the first portion contributes to the membrane binding of Cterminal-RDH8.Bovine and human retinol dehydrogenase 8,respectively contain 312 and 331 amino acid residues and can only be found in photoreceptors. |
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| Significance in milk | bovine retinal pigment formation |
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| PTMs | Palmitoylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| NA |
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | no TM helices |
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| Significance of PTMs | Palmitoylation of at least one of the cysteine residues in the C terminus(Cys-299,Cys-302,Cys-304) results in the membrane binding of RdH8. |
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| Bibliography | 1.Hädicke A, Coutinho A, Roy S, Otis F, Lhor M, Cantin L, Prieto M, Voyer N, Salesse C. Membrane binding properties of the C-terminal segment of retinol dehydrogenase 8. Biochim Biophys Acta Biomembr. 2021 Sep 1;1863(9):183605. doi: 10.1016/j.bbamem.2021.183605. Epub 2021 Mar 22. PMID: 33766534. |
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