Search by BoMiProt ID - Bomi8560

Primary Information

BoMiProt ID Bomi8560
Protein Name Quinone oxidoreductase-like protein 1/Quinone oxidoreductase homolog 1/Zeta-crystallin homolog
Organism Bos taurus
Uniprot IDQ59A28
Milk FractionWhey
Ref Sequence ID NP_001030209.2
Aminoacid Length 349
Molecular Weight 38812
FASTA Sequence Download
Gene Name CRYZL1
Gene ID 506426
Protein Existence Status reviewed

Secondary Information

Protein Function zeta-Crystallin, a major cytosolic protein of guinea pig lens, has been characterized as an NADPH:quinone oxidoreductase
Biochemical Properties A bovine lens homolog with 83% sequence identity was found to have very different functional characteristics. While the bovine lens zeta-crystallin exhibits similar physicochemical properties, such as molecular weight, hydropathy profile, and predicted secondary structure, and exhibits strong immunological cross-reactivity with the guinea pig and human lens zeta-crystallins, it shows minimal quinone oxidoreductase activity. 
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments loss of a functional residue accounts for the lowered catalytic activity of the bovine protein, Tyr 52 of the E. coli enzyme, and the equivalent Tyr present in all known mammalian forms except the bovine, is the likely candidate. In the bovine form this tyrosine is replaced by histidine.
Bibliography 1.Rao PV, Gonzalez P, Persson B, Jörnvall H, Garland D, Zigler JS Jr. Guinea pig and bovine zeta-crystallins have distinct functional characteristics highlighting replacements in otherwise similar structures. Biochemistry. 1997 May 6;36(18):5353-62. doi: 10.1021/bi9622985. PMID: 9154917.