Primary Information |
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BoMiProt ID | Bomi8403 |
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Protein Name | Protein PHTF1 |
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Organism | Bos taurus |
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Uniprot ID | Q08DA4 |
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Milk Fraction | Whey |
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Ref Sequence ID | NP_001069545.1 |
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Aminoacid Length | 762 |
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Molecular Weight | 87159 |
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FASTA Sequence |
Download |
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Gene Name | PHTF1 |
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Gene ID | 536504 |
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Protein Existence Status | reviewed |
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Secondary Information |
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Protein Function | Phtf1 provides a potential new marker for Golgi modifications as well as for many of the obscure transformations undergone by the endoplasmic reticulum. It could help to elucidate the morphogenic events connected with the transformation of spermatogenic cells. |
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Biochemical Properties | The conservation between human (PHTF1) and mouse (phtf1) protein extends over the entire protein and is remarkably high (98% similarity). We reported previously the cloning of the Drosophila ortholog (d-phtf) that displays 56% similarity with phtf1 over its entire length. The highest identities between PHTF1 and d-phtf were localized at the NH2- and COOH-terminal regions and included a putative homeodomain region. However, 7–8 stretches of hydrophobic amino acid residues could indicate that phtf1 is a membrane-spanning protein. |
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PTMs | N-Linked Glycosylation at Asn and Phosphorylation at Ser |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q08DA4|PHTF1_BOVIN Protein PHTF1 OS=Bos taurus OX=9913 GN=PHTF1 PE=2 SV=1
MASNERDAISWYQKKIGAYDQQIWEKSIEQTQIKGFKNKPKKMGHIKADLIDVDLIRGST
FAKAKPEIPWTSLTRKGLVRVVFFPLFSSWWIQVTSLRIFVWLLLLYLMQVIALVLYFMM
PIVNVSEVLGPLCLMLLMGTVHCQIVSTQITRPSGNNGNRRRRKLRKTVNGDGSRENGN*179N*180
SSDKARGVETLESAPLN*197GSFWRTLFGNRMKRVKLICNRGTETDYDSGCLHPIIKKRQCRP
EIRMWQTREKAKFSDGEKGRRESFRRLGNGIS*272DDLS*276S*277DDDGEAQTQMMILRRSVEGASSD
NGCEIKSRKSILSRHLNTQVKKTTSKWCSVVRDS*334DS*336LAESEFESAAFSQGSRSGMSGGSR
SLNMLRRDSESTRHDSETEDMLWDDLLHGPECRSSVTSDSEGAHVSSLHSGTKRDPKEDV
FQQNHLFWLQN*431SSPASDRVSAIIWEGNECKKMDMSVLEISGIIMSRVNAYQQGVGYQMLG
NIVTIGLAFFPFLHRLFREKNLDQLKSISAEEILTLFCGAPPVTPIIILSIINFFERLCL
TWMFFFMMCVAERTYKQRFLFAKLFSHITSARKARKYEIPHFRLKKVENIKIWLSLRSFL
KRRGPQRSVDVVVSSVFLLTLSIAFICCAQVLRGHKTFLNDAYNWEFLIWESALLLFLLR
LASLGSETNKKYSN*674VSILLTEQINLYLKMEKKPNKKEQLTLVNNVLKLSTKLLKELDTPF
RLYGLTMNPLIYN*733ITRVVILSAVSGVISDLLGFNIRLWKIKS
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Predicted Disorder Regions | 153-188, 248-283, 299-316, 340-378, 400-413 |
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DisProt Annotation | |
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TM Helix Prediction | 7TMHs; (100-122),(128-150),(472-494),(513-535),(611-633),(648-666),(737-755) |
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Additional Comments | Phtf1 possesses an evolutionarily-conserved structure and also displays a conserved pattern of expression even in Drosophila (data not shown), which strongly suggests that this protein may play an important role in the differentiation of germinal cells. |
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Bibliography | 1.Oyhenart J, Le Goffic R, Samson M, Jégou B, Raich N. Phtf1 is an integral membrane protein localized in an endoplasmic reticulum domain in maturing male germ cells. Biol Reprod. 2003 Mar;68(3):1044-53. doi: 10.1095/biolreprod.102.009787. PMID: 12604659. 2.Manuel A, Beaupain D, Romeo PH, Raich N. Molecular characterization of a novel gene family (PHTF) conserved from Drosophila to mammals. Genomics 2000; 64:216–220. |