Primary Information |
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| BoMiProt ID | Bomi8372 |
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| Protein Name | Protein NEDD1/Neural precursor cell expressed developmentally down-regulated protein 1 |
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| Organism | Bos taurus |
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| Uniprot ID | Q3B7M6 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001030410.1 |
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| Aminoacid Length | 659 |
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| Molecular Weight | 71204 |
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| FASTA Sequence |
Download |
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| Gene Name | NEDD1 |
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| Gene ID | 519463 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Promotes the nucleation of microtubules from the spindle and spindle assembly.Interacts with γ-TuRC, a γ-tubulin complex. |
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| Biochemical Properties | contains γ-tubulin-binding domain. |
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| PTMs | Phosphorylation on Ser |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q3B7M6|NEDD1_BOVIN Protein NEDD1 OS=Bos taurus OX=9913 GN=NEDD1 PE=2 SV=1
MQENLRFASSGDDVKIWDASSMTLVDKFNPHTAPHAISSVCWSSNNNFLVTASSSGDKIV
VSSCKCKPVPLLELGEGQKQTCVSLNSTSMYLVSGGLNNTVNIWDLKSKRVHRSLKDHKD
EVTCVTYNWNDCYIASGSLSGEIILHSVTTNLSSTPFGHGSNQSIRHLKYSLFKKSLLGS
VSDNGIVTLWDVNSQSPYHNFDSTHKAPASGICFSPVNELLFVTVGLDKRIILYDTSSKK
LVKTLVADAPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPIKTISAHKTSVQCIAFQ
YSTVLSKSGLNKGCSNKPTAVNKRTANVSAGGGGAQNPGVVREAATTSIATVPPQPTAAA
VGKGAVAPQDKAGLPRSINTDT*382LSKEAESGKNQDFSNFDDSGKSSLGDMFS*411PVRDDAVVS
KGGDES*426IGKGDGLDFLPQLNSVFPPRKNPVVSSTSVLHSSPLNVFMGS*468PGKEENENHDLT
AESKKMYLGKQESKDSFKQFAKLISGAETGNLNAS*515PSSNQTRSPEKFEKPEKEIEAQLIN
EPPGNGSST*549PNPKIASSVTAGVAGSLSEKIVDTIGNSRPNAPLSSVQIRFIQNMIQETLD
DFREACHRDIVNLQVEMIKQFHMQLNEMHSLLERYS*636VNEGLVAEIERLREENKRLRAHF
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| Predicted Disorder Regions | 343-410, 423-448, 467-580 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | There are multiple novel phosphorylated sites in the serine (Ser)557-S574 region of NEDD1, close to its γ-tubulin-binding domain.Phosphorylation in the S557–T560 region has a negative effect on NEDD1 function.phosphorylation of the S565–S574 region may promote NEDD1 activity.During mitosis,phosphorylation on Thr-549 by CDK1 which furthur promotes phosphorylation by PLK1 on Thr-382, Ser-426 and Ser-636. |
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| Bibliography | Gomez-Ferreria MA, Bashkurov M, Helbig AO, Larsen B, Pawson T, Gingras AC, Pelletier L. Novel NEDD1 phosphorylation sites regulate γ-tubulin binding and mitotic spindle assembly. J Cell Sci. 2012 Aug 15;125(Pt 16):3745-51. doi: 10.1242/jcs.105130. Epub 2012 May 17. PMID: 22595525. |
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