Primary Information |
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| BoMiProt ID | Bomi8319 |
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| Protein Name | Protein argonaute-3/Argonaute3/Argonaute RISC catalytic component 3/Eukaryotic translation initiation factor 2C 3/eIF-2C 3/
eIF2C 3 |
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| Organism | Bos taurus |
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| Uniprot ID | Q6T5B7 |
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| Milk Fraction | Whey |
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| Aminoacid Length | 861 |
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| Molecular Weight | 97300 |
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| FASTA Sequence |
Download |
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| Gene Name | AGO3/EIF2C3 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | essential for piRNA amplification, have endonuclease activity.needed for proper germline development. |
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| Biochemical Properties | PAZ domain, the MID domain in the middle region, and the PIWI domain in the C terminus.PAZ and MID domains are proposed to bind to the 3′ and 5′ termini of a small RNA, respectively.PIWI domain possesses the conserved catalytic motif Asp-Asp-His (D-D-H: 643D, 713D, and 842H) which shows slicer activity. |
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| PTMs | Hydroxylation,Methylation,ubl conjugation,Phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q6T5B7|AGO3_BOVIN Protein argonaute-3 OS=Bos taurus OX=9913 GN=AGO3 PE=2 SV=2
MEIGSAGPVGAQPLLMVPRRPGYGTMGKPTKLLANCFQVEIPKIDVYLYEVDIKPDKCPR
RVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDATLPGEGGKDRP
FKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGR
SFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLD
IHNIDEQPRPLTDSHRVKSTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLE
NGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDN
QTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQ
YGGRNRTVATPSHGVWDMRGKQFHTGVEIEMWAIACFATQRQCREEILKGFTDQLRKISK
DAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTL
LGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVT
HPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRF
KPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCAD
RTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEL
QLLTYQPSAHTYVHCTRSVSIPAPAYYAHLVAFRARYHLVDKERDS*826AEGSHVSGQSNGRD
PQALAKAAQIHQDTLRTMYFA
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| Predicted Disorder Regions | 824-842 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | prolyl 4-hydroxylation by type I collagen prolyl 4-hydroxylase (C-P4H9I) on AGO3,probably necessary for stability.prolyl 4-hydroxylation appears to be dispensable for miRNA-mediated translation repression. methyltransferase 5 (dPRMT5) catalyses the methylation of Ago3 and Aubergine, which enhances their stability.Methylation of Piwi proteins facilitates the recruitment of multiple members of the Tudor family, which may assist in piRNA production and loading of Piwi protein.Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex during degradation of microRNAs (miRNAs). |
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| Bibliography | Huang H, Li Y, Szulwach KE, Zhang G, Jin P, Chen D. AGO3 Slicer activity regulates mitochondria-nuage localization of Armitage and piRNA amplification. J Cell Biol. 2014 Jul 21;206(2):217-30. doi: 10.1083/jcb.201401002. PMID: 25049272; PMCID: PMC4107788. |
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