Primary Information |
|---|
| BoMiProt ID | Bomi8217 |
|---|
| Protein Name | Probable G-protein coupled receptor 158 |
|---|
| Organism | Bos taurus |
|---|
| Uniprot ID | E1BBQ2 |
|---|
| Milk Fraction | Whey |
|---|
| Aminoacid Length | 1216 |
|---|
| Molecular Weight | 135363 |
|---|
| FASTA Sequence |
Download |
|---|
| Gene Name | GPR158 |
|---|
| Protein Existence Status | reviewed |
|---|
Secondary Information |
|---|
| Protein Function | Predicted to enable G protein-coupled receptor activity. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and protein localization to plasma membrane. Predicted to be located in membrane. |
|---|
| Biochemical Properties | Most Family C GPCRs containan N-terminal Venus Fly Trap (VFT) domain that is linked to the 7TM domain via the cysteine-rich domain (CRD) and plays an important role in ligand recognition. While GPPR158 lacks the VFT domain.GPR158 contains several potential Nglycosylation sites, all of them located in the N-terminal domain, but no O-glycosylation sites. |
|---|
| PTMs | N-Linked Glycosylation at Asn, Isopeptide bond formation, Phosphorylation at Ser, Ubl conjugation |
|---|
Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|E1BBQ2|GP158_BOVIN Probable G-protein coupled receptor 158 OS=Bos taurus OX=9913 GN=GPR158 PE=3 SV=2
MGVMAYPFLFCLLLVHFGLGAIGASREAPSRPDPPRERTLRAKQHAQQPARASASDPSAP
WSRSTDGTILAQKLAEEVPMDVASYLYTGDSHKLKRAN*98CSSRYELAGLPGKSPALASSHP
SLHGALDTLTHATNFLNMMLQSN*143KSREQNLQDDLEWYQALVRSLLEGEPSISRAAITFST
ESLSAPAPQVFLQATREESRILLQDLSSSAHHLAN*215ATLETEWFHGLRRKWRTHLHRRGSN
QGPRGLGHSWRRRDGLSGDKSHVKWSPPYLECEN*274GSYKPGWLVTLSAAFYGLQPNLVPEF
RGVMKVDINLQKVDIDQCSSDGWFSGTHKCHLN*333NSECMPIKGLGFVLGAYQCICKAGFYH
PRDFSFNNFQRRGPDHHFVESAKDVSEEVHVCLPCSEGCPYCADDSPCFVQEDKYLRLAI
ISFQALCMLLDFLSMLVVYRFRKAKSIRASGLILLETILFGSLLLYFPVVILYFEPSTFR
CILLRWVRLLGFATVYGTVTLKLHRVLKVFLSRTAQRIPYMTGGRVMRMLAVILLVVFWF
LVGWTSSVCQNLERHISLIGQGRTSDHLIFSMCLVERWDYMTAAAEFLFLLWGVYLCYAV
RTVPSAFHEPRYMAVAVHNELIISAIFHTIRFVLASRLQSDWMLMLYFAHTHLTVTVTIG
LLLIPKFSHSSNNPRDDIATEAYEDELDMGRSGS*694YLNSSINSAWS*705EHS*708LDPEDIRDELKK
LYAQLEIYKRKKMITNNPHLQKKRCSKKGLGRSIMRRITEIPETVSRQCSKEDKDGGEHG
SAKGSSAGRKNPQETAGGSGKPKEESLRSRVFSLKKSHSTYEHVREQPGGPGSPPAQSRE
EEEATDNSVLGSPVGANRPRPLQEDS*866QAVSTESVPLVCKSASAHNLSSEKKPGPTRTSVL
QKSLSVIASAKEKTLGLAGKTQAACVEERAKAQKALPRERETNRKYS*947NSDNAETQDSAPP
NSSHSEEPRKPQKLGIMKQQRANPTTANSDLSPGATHMKDNFDIGEVCPWEIYDLAPGPG
PLESKVQKHVSIAASEMERNPTFSLKEKSHPKPKAADLCQQSNPKS*1066VDKAEVCPWESQGQ
S*1081LFEEEKYLMSKTQVPLGRAQGENSGQHCATGVCAGQCEELPPKAVASKVENENLNQLGE
QEKKTSSSERNVPDSHNSSNNFQPPLMSRAEVCPWEFETPDKPNAERSVAFPASSALSAN
KIAGPRNEEVRLARKV
|
|---|
| Predicted Disorder Regions | 27-62, 741-751, 774-1201 |
|---|
| DisProt Annotation | |
|---|
| TM Helix Prediction | 7TMHs; (3-25), (421-439),(452-474),(489-507),(530-548),(613-635),(642-664) |
|---|
| Additional Comments | GPR158 overexpression upregulates levels of the cell cycle regulator cyclin D1, but mutation of the NLS reverses this. Overexpression of GPR158 enhances the barrier function of a TBM cell monolayer, which is associated with an increase in the levels of tight junction proteins ZO-1 and occludin, similar to reported studies on GC treatment |
|---|
| Bibliography | 1.Patel N, Itakura T, Gonzalez JM Jr, Schwartz SG, Fini ME. GPR158, an orphan member of G protein-coupled receptor Family C: glucocorticoid-stimulated expression and novel nuclear role. PLoS One. 2013;8(2):e57843. doi: 10.1371/journal.pone.0057843. Epub 2013 Feb 25. PMID: 23451275; PMCID: PMC3581496. 2.Wellendorph P, Bra¨uner-Osborne H (2009) Molecular basis for amino acid
sensing by family C G-protein-coupled receptors. Br J Pharmacol 156:869–884. 3.Kniazeff J, Pre´zeau L, Rondard P, Pin JP, Goudet C (2011) Dimers and beyond:
The functional puzzles of class C GPCRs. Pharmacol Ther 130:9–25 |
|---|
