Primary Information |
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| BoMiProt ID | Bomi8135 |
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| Protein Name | Polycystin-2/Polycystic kidney disease 2 protein homolog/Transient receptor potential cation channel subfamily P member 2 |
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| Organism | Bos taurus |
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| Uniprot ID | Q4GZT3 |
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| Milk Fraction | Whey |
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| Aminoacid Length | 970 |
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| Molecular Weight | 109789 |
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| FASTA Sequence |
Download |
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| Gene Name | PKD2 |
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| Gene ID | 530393 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | transient receptor potential channel |
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| Biochemical Properties | contains 6 transmembrane regions, a pore-forming loop, N-glycosylation and phosphorylation sites, a polycystin-specific ‘tetragonal opening for polycystins’ (TOP) domain,and a C-terminal tail that includes a Ca2+-binding EF hand, coiled-coil domain, and ER retention tag. |
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| PTMs | phosphorylation and glycosylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q4GZT3|PKD2_BOVIN Polycystin-2 OS=Bos taurus OX=9913 GN=PKD2 PE=2 SV=1
MVNSSRVQPQQPGDARRSPAPRAPGPGRLMAGGAIAGAGLAAPGGLREQRGLEIEMERIR
QAAARDPPAGASASPS*76PPLS*80SCSRQAWSRDNPGFEAEEEEEEEEVEGEEGGMVVEMDVEW
RPGSRRSASSSAVSSAGARGRGLGGYHGAGHPSGRRRQREDQGPPSPSPAGGGDPLHRHL
PLDGQHPRVAWAERLVRGLRGLWGTRLMEESSTDREKYLKSVLRELATYLLFLIVLCILT
YGMMSSSVYYYTRIMSQLFLDTPVSKMEKTNFKTLSSMEDFWKFTEGALLDGLYWKTQPS
N*301RTEADN*307RSFIYYENLLLGVPRIRQLRVRN*330GSCSIPLDLRDEIKECYDVYSVSSEDRAPF
GPRN*364GTAWIYTSEKDLN*377GSSHWGMIATYSGAGYYLDLSRTREETAAQVANLKKNVWLDRG
TRAIFIDFTVYNANINLFCVIRLLIEFPATGGVIPSWQFQPVKLIRYVTTFDFFLAACEI
IFCLFILYYVVEEILEIRIHKLHYFRSFWNCLDVVIIVLSVVAIGINIYRTSNVEALLQF
LEDQNTFPNFENLAYWQTQFNNIAAVIVFFVWIKLFKFINFNRTMSQLSTTMSRCAKDLF
GFAIMFFIIFLAYAQLAYLVFGTQVDDFSTFQECIFTQFRIILGDINFAEIEEANRVLGP
IYFTTFVFFMFFILLNMFLAIINDTYSEVKSDLAQQKAEMELSDLIRKGYHKALIKLKLK
KNTVDDISESLRQGGGKLNFDELRQDLKGKGHTDAEIEAIFTKYDQDGDQELTEHEHQQM
RDDLEKEREDLDLDHSSLPRPMS*803SRSFPRS*810LDDS*814EEEDDDDSGHSSRRRGS*831ISSGVSYEEFQVLVRRVDRMEHSIGSIVSKIDAVIVKLEIMERAKLKRREVLGRLLDGVAEDERLGRDN
EIHREQMERLVREELERWESDDAASQISHGLGTPLGLNGQPRPRSSRPSSSQSTEGMEGG
GGNGSANIHV
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| Predicted Disorder Regions | 1-20, 68-140, 144-165, 766-833, 906-969 |
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| DisProt Annotation | |
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| TM Helix Prediction | 7TMHs; (227-249), (432-454), (473-491), (504-526), (563-581), (599-621), (660-682) |
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| Significance of PTMs | Ser801 phosphorylation by protein kinase D (PrKD) is required for proper cell proliferation and inositol trisphosphate (InsP3)-mediated ER Ca2+ release.Constitutive Ser812 phosphorylation by casein kinase 2 (CK2) is critical to maintain proper Ca2+ dependence of PC2 along with its retrograde transport.Ser829 phosphoserine site is also thought to enhance InsP3-mediated Ca2+ transients. Phosphorylation and dephosphorylation of Ser534 by CK2 and calcineurin (CaN), respectively, mediates PC2 ciliary localization in C. elegans.the N-glycosylation of five residues within the expansive TOP domain of PC2 (amino acids: Asn299, 305, 328, 362, and 375).In its multiple-ion (inhibited) state, Asn299, 328, 362 and 375 are N-glycosylated, and are important for maintaining well-ordered loop interactions. The N-glycosylation of Asn305 is predicted to prevent the intra-tetramer interactions that occur in the multiple-ion state, thus leaving the single-ion state less ordered. |
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| Bibliography | Brill AL, Ehrlich BE. Polycystin 2: A calcium channel, channel partner, and regulator of calcium homeostasis in ADPKD. Cell Signal. 2020 Feb;66:109490. doi: 10.1016/j.cellsig.2019.109490. Epub 2019 Dec 2. PMID: 31805375; PMCID: PMC6935422. |
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