Search by BoMiProt ID - Bomi8001

Primary Information

BoMiProt ID Bomi8001
Protein Name Phospholipase D2
Organism Bos taurus
Uniprot IDQ0V8L6
Milk FractionWhey,MFGM
Ref Sequence ID NP_001069295.1
Aminoacid Length 933
Molecular Weight 105766
FASTA Sequence Download
Gene Name PLD2
Gene ID 522159
Protein Existence Status reviewed

Secondary Information

Protein Function PLD2 promotes PTPN14-mediated dephosphorylation of VE-cadherin and that redistribution of VE-cadherin at adherens junctions is essential for recovery of endothelial barrier function after an edemagenic insult.
Biochemical Properties PLD generated PA mediates a variety of cellular functions, including growth/proliferation, vesicle trafficking, cytoskeletal rearrangement and activation of NADPH Oxidase (NOX), and most of its functions are attributed to the broad interactions with other proteins and lipids.
PTMs Phosphorylation and Ubiquitinated at Lys-454,466 residue
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions 1-36, 139-156
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs RNF145 triggers ubiquitination of SCAP, likely inhibiting SCAP-SREBP complex and transport it to the Golgi apparatus and the subsequent maturation of SREBF2/SREBP2.
Additional Comments PLD2 protein co-expresses with chromogranin A in castrate-resistant prostate cancer tissue. PLD2 inhibition reduces prostate cancer cell viability, colony forming ability and directional cell movement.
Bibliography 1.Fu P, Ramchandran R, Shaaya M, Huang L, Ebenezer DL, Jiang Y, Komarova Y, Vogel SM, Malik AB, Minshall RD, Du G, Tonks NK, Natarajan V. Phospholipase D2 restores endothelial barrier function by promoting PTPN14-mediated VE-cadherin dephosphorylation. J Biol Chem. 2020 May 29;295(22):7669-7685. doi: 10.1074/jbc.RA119.011801. Epub 2020 Apr 23. PMID: 32327488; PMCID: PMC7261798. 2.Noble AR, Hogg K, Suman R, Berney DM, Bourgoin S, Maitland NJ, Rumsby MG. Phospholipase D2 in prostate cancer: protein expression changes with Gleason score. Br J Cancer. 2019 Dec;121(12):1016-1026. doi: 10.1038/s41416-019-0610-7. Epub 2019 Nov 1. PMID: 31673104; PMCID: PMC6964697.