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Primary Information | |
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| BoMiProt ID | Bomi7939 |
| Protein Name | Phosphatidylinositol 3-kinase regulatory subunit beta/PI3-kinase regulatory subunit beta/PI3K regulatory subunit beta/PtdIns-3-kinase regulatory subunit beta/Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta/PI3-kinase subunit p85-beta/ PtdIns-3-kinase regulatory subunit p85-beta |
| Organism | Bos taurus |
| Uniprot ID | P23726 |
| Milk Fraction | Whey |
| Ref Sequence ID | NP_777001.1 |
| Aminoacid Length | 724 |
| Molecular Weight | 81060 |
| FASTA Sequence | Download |
| Gene Name | PIK3R2 |
| Gene ID | 282308 |
| Protein Existence Status | reviewed |
Secondary Information | |
| Protein Function | regulatory subunit of phosphoinositide-3-kinase (PI3K) which converts PI(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3),enables the recruitment to the plasma membrane and activation of downstream proteins that contain lipid-binding domains like pleckstrin homologue (PH) domain and the phox homolog (PX) domain. |
| Biochemical Properties | contains an N-terminal SH3 region, 2 SH2 regions, and a region of homology to BCR. lack intrinsic kinase activity, |
| PTMs | phosphorylation on Tyr,Ubl conjugation |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|P23726|P85B_BOVIN Phosphatidylinositol 3-kinase regulatory subunit beta OS=Bos taurus OX=9913 GN=PIK3R2 PE=1 SV=1 MAGPEGFQYRALYPFRRERPEDLELLPGDVLVVSRAALQALGVAEGNERCPQSVGWMPGL NERTRQRGDFPGTYVEFLGPVALARPGPRPRGPRPLPARPRDGPPEPGLTLPDLPEQFSP PDVAPPILVKLVEAIERTGLDSYRPEPPAVRTDWSLSDVEQWDAAALSDGVKGFLLALPA PLVTPEAAAEAHRALREAAGPVGPALEPPTLPLHHALTLRFLLQHLGRVAGRAPAPGPAV RALGATFGPLLLRAPPPPSPPPGGAPDGTEPTPDFPALLVEKLLQEHLEEQEVAPPALPP KPPKTKPAPTGLANGGSPPSLQDAEWYWGDISREEVNEKLRDTPDGTFLVRDASSKIQGE YTLTLRKGGNNKLIKVFHRDGHYGFSEPLTFCSVVDLITHYRHESLAQYNAKLDTRLLYP VSKYQQDQIVKEDSVEAVGAQLKVYHQQYQDKSREYDQLY*460EEYTRTSQELQMKRTAIEAF NETIKIFEEQGQTQEKCSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQ ELRAQASDNREIDKRMNSLKPDLMQLRKIRDQYLVWLTQKGARQKKINEWLGIKNETEDQ Y*601SLMEDEDDLPHHEERTWYVGKINRTQAEEMLSGKRDGTFLIRESSQRGCY*651ACSVVVDGD TKHCVIYRTATGFGFAEPYNLYGSLKELVLHYQHASLVQHNDALTVTLAHPVRAPGPGPP PAAR |
| SCOP | Class : All alpha proteins Fold : Left-handed antiparallel coiled-coil Superfamily : PI3K inter-SH2 coiled-coil domain Family : PI3K inter-SH2 coiled-coil domain Domain Name : 3L4Q C:429-591 |
| CATH | Matched CATH superfamily 1.10.287.1490 |
| Predicted Disorder Regions | NA |
| DisProt Annotation | |
| TM Helix Prediction | No TM helices |
| Significance of PTMs | Phosphorylated in response to signaling from activated receptor-type protein kinases which is imp for various cellular process.Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.FBXL2-mediated degradation of PI3K regulatory subunit beta is imp for its regulation during signaling cascade.phosphorylation of p85β on Tyr 655, which is adjacent to the degron, inhibits p85β binding to FBXL2.Dephosphorylation of phospho-Tyr-655 by PTPL1 stimulates p85β binding to and degradation through FBXL2. |
| PDB ID | 3L4Q, |
| Additional Comments | mutations are the driving factors of two epilepsy-related developmental brain disorders, megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome (MPPH) and bilateral perisylvian polymicrogyria (BPP). |
| Bibliography | Kuchay S, Duan S, Schenkein E, Peschiaroli A, Saraf A, Florens L, Washburn MP, Pagano M. FBXL2- and PTPL1-mediated degradation of p110-free p85β regulatory subunit controls the PI(3)K signalling cascade. Nat Cell Biol. 2013 May;15(5):472-80. doi: 10.1038/ncb2731. Epub 2013 Apr 21. PMID: 23604317; PMCID: PMC3865866. |