Primary Information |
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| BoMiProt ID | Bomi7877 |
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| Protein Name | Peptidyl-glycine alpha-amidating monooxygenase/Peptidylamidoglycolate lyase |
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| Organism | Bos taurus |
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| Uniprot ID | P10731 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_776373.1 |
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| Aminoacid Length | 972 |
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| Molecular Weight | 108177 |
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| FASTA Sequence |
Download |
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| Gene Name | PAM |
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| Gene ID | 280890 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Catalyzes the post-translational modification of inactive peptidylglycine precursors to bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neuropeptide hormones. |
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| Biochemical Properties | Reaction 1-peptidylglycine is converted to peptidyl alpha hydroxy glycine by PHM(peptidylglycine a-hydroxylating monooxygenase), which furthur converted to peptide alpha amide and glycoxylate.1st reaction requires copper,O2,ascorbate. And 2nd reaction is catalysed by peptidyl-a-hydroxyglycine alpha-amidating lyase(PAL) in alkaline pH. |
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| Significance in milk | Peptide amidation imp for milk protein synthesis |
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| PTMs | phosphorylation on Ser and Thr |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|P10731|AMD_BOVIN Peptidyl-glycine alpha-amidating monooxygenase OS=Bos taurus OX=9913 GN=PAM PE=1 SV=2
MAGFRSLLVLLLVFPSGCVGFRSPLSVFKRFKETTRSFSNECLGTTRPVIPIDSSDFALD
IRMPGVTPKQSDTYFCMSVRLPMDEEAFVIDFKPRASMDTVHHMLLFGCNMPASTGNYWF
CDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKD
CSGVSLHLTRLPQPLIAGMYLMMSVDTVIPPGGKVVNSDISCHYKKYPMHVFAYRVHTHH
LGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVEHPVDVSFGDILAARCVFTGEGRTEVTH
IGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDIFRTIPPEANIPIPVKSDMVMMHG
HHKETENKDKTSLLQQPKREEEGVLEQGDFYSLLSKLLGEREDVVHVHKYNPTEKAESES
DLVAEIANVVQKKDLGRSDTRESAEQERGNAILVRDRIHKFHRLVSTLRPAESRVLSLQQ
PLPGEGTWEPEHTGDFHVEEALDWPGVYLLPGQVSGVALDPQNNLVIFHRGDHVWDGNSF
DSKFVYQQRGLGPIEEDTILVIDPNNAAVLQSSGKNLFYLPHGLSIDKDGNYWVTDVALH
QVFKLDPKSKEGPLLTLGRSMQPGSDQNHFCQPTDVAVDPDTGTIYVSDGYCNSRLVQFS
PSGKFITQWGEASLESSPKPGQFRVPHSLALVPPLGQLCVADRENGRIQCFKTDTKEFVR
EIKHPSFGRNVFAISYIPGLLFAVNGKPYFEDQEPVQGFVMN*762FSSGEIIDVFKPVRKHFD
MPHDIAASEDGTVYVGDAHTNTVWKFTSTEKMEHRSVKKAGIEVQEIKESEAVVETKMEN
KPASSELQKIQEKQKLVKEPGSGVPAVLITTLLVIPVVVLLAIALFIRWKKSRAFGDSER
KLEASSGRVLGRLRGKGGGGLNLGNFFAS*929RKGYSRKGFDRLS*942T*943EGS*946DQEKDEDASES*957EEEYSAPPPAPAPSS
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| Predicted Disorder Regions | 926-972 |
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| DisProt Annotation | |
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| TM Helix Prediction | 1TMH; (865-887) |
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| Bibliography | Eipper BA, Milgram SL, Husten EJ, Yun HY, Mains RE. Peptidylglycine alpha-amidating monooxygenase: a multifunctional protein with catalytic, processing, and routing domains. Protein Sci. 1993 Apr;2(4):489-97. doi: 10.1002/pro.5560020401. PMID: 8518727; PMCID: PMC2142366. |
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