Primary Information
BoMiProt ID	Bomi7472
Protein Name	Neutral cholesterol ester hydrolase 1/Arylacetamide deacetylase-like 1/NCEH
Organism	Bos taurus
Uniprot ID	Q1JQE6
Milk Fraction	Whey
Ref Sequence ID	NP_001116506.1
Aminoacid Length	408
Molecular Weight	46026
FASTA Sequence	Download
Gene Name	NCEH1/AADACL1
Gene ID	534212
Protein Existence Status	reviewed
Secondary Information
Protein Function	NCEH1 is expressed in human atheromatous lesions, where it plays a critical role in the hydrolysis of CE in human macrophage foam cells, thereby contributing to the initial part of reverse cholesterol transport in human atherosclerosis.
Biochemical Properties	hree enzymes have been proposed to serve as nCEHs in macrophages: hormone-sensitive lipase (LIPE); cholesteryl ester hydrolase (CEH), which is identical to human liver carboxylesterase1 (CES1, hCE-1) or macrophage serine esterase1 (HMSE1), also known as a human ortholog of triacylglycerol hydrolase (TGH); and neutral cholesterol ester hydrolase 1(Nceh1, MGI ID 2443191), which is also known as KIAA1363 or arylacetamide deacetylase-like 1 (Aadacl1).
PTMs	N-glycosylated
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|Q1JQE6|NCEH1_BOVIN Neutral cholesterol ester hydrolase 1 OS=Bos taurus OX=9913 GN=NCEH1 PE=2 SV=2 MRSSCVLLTALLALAAYYIYIPLPSSVSDPWKLMLLDATFRSAQQVSNLIHFLGLSHHLL ALNFIIVSFGKKSAWSSAQVKVTDTDFDGVEVRVFEGPPKPEEPLKRSIVYIHGGGWALA SAKIRYYDELCTTMAEELNAVIVSIEYRLVPKVYFPEQIHDVVHATKYFLQPEVLHKYSV DPGRVGISGDSAGGNLAAALGQQFNQDTNLKNKLKVQALIYPVLQALDFNTPSYQQNMNT PILPRYVMVKYWVDYFNGNYDFVQAMIVNN*270HTSLDVDEASALRARLN*287WTSLLPTSITKNY KPVMQTTGNSRIVQEIPQLLDARSAPLIADQEVLQHLPKTYILTCEHDVLRDDGIMYAKR LESAGVEVTLDHFEDGFHGCMIFTSWPTN*389FSVGIRTRNSYIKWLDQNL
Predicted Disorder Regions	NA
DisProt Annotation
TM Helix Prediction	1TMH; (7-25)
Additional Comments	Overexpression of human NCEH1 increased the hydrolysis of CE, thereby stimulating cholesterol mobilization from THP-1 macrophages.
Bibliography	1.Igarashi M, Osuga J, Uozaki H, Sekiya M, Nagashima S, Takahashi M, Takase S, Takanashi M, Li Y, Ohta K, Kumagai M, Nishi M, Hosokawa M, Fledelius C, Jacobsen P, Yagyu H, Fukayama M, Nagai R, Kadowaki T, Ohashi K, Ishibashi S. The critical role of neutral cholesterol ester hydrolase 1 in cholesterol removal from human macrophages. Circ Res. 2010 Nov 26;107(11):1387-95. doi: 10.1161/CIRCRESAHA.110.226613. Epub 2010 Oct 14. PMID: 20947831. 2.Sekiya M, Osuga J, Nagashima S, Ohshiro T, Igarashi M, Okazaki H, Takahashi M, Tazoe F, Wada T, Ohta K, Takanashi M, Kumagai M, Nishi M, Takase S, Yahagi N, Yagyu H, Ohashi K, Nagai R, Kadowaki T, Furukawa Y, Ishibashi S. Ablation of neutral cholesterol ester hydrolase 1 accelerates atherosclerosis. Cell Metab. 2009 Sep;10(3):219-28. doi: 10.1016/j.cmet.2009.08.004. PMID: 19723498.