Search by BoMiProt ID - Bomi7363

Primary Information

BoMiProt ID Bomi7363
Protein Name NAD-dependent protein deacylase sirtuin-5, mitochondrial/Regulatory protein SIR2 homolog 5/SIR2-like protein 5
Organism Bos taurus
Uniprot IDQ3ZBQ0
Milk FractionWhey
Ref Sequence ID NP_001029467.1
Aminoacid Length 310
Molecular Weight 33988
FASTA Sequence Download
Gene Name SIRT5
Gene ID 507347
Protein Existence Status reviewed

Secondary Information

Protein Function primarily in the mitochondrial matrix, NAD+-dependent protein deacylases/ADP-ribosyltransferases and catalyzes the removal of negatively charged lysine acyl modifications; succinyl, malonyl, and glutaryl groups.SIRT5 mediated desuccinylation is imp for maintaining cardiac energy metabolism.promotes the activity of GAPDH and perhaps other glycolytic enzymes via demalonylation.desuccinylates PKM2 at lysine 311 to increase pyruvate kinase activity
Biochemical Properties The N-terminus contains a 36 amino acid canonical mitochondrial localization signal (MLS) and an additional stretch of 72 aa , is present in isoforms 1, 2 and 3, but absent in isoform 4.Consume nicotinamide adenine dinucleotide (NAD+) as a co-substrate, and generate the sirtuin feedback inhibitor nicotinamide (NAM), 2’-O-acyl (succinyl/malonyl/glutaryl)-ADP-ribose, and a deacylated substrate.
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments  Sirt5 KO mice display reduced glycolytic flux 
Bibliography Kumar S, Lombard DB. Functions of the sirtuin deacylase SIRT5 in normal physiology and pathobiology. Crit Rev Biochem Mol Biol. 2018 Jun;53(3):311-334. doi: 10.1080/10409238.2018.1458071. Epub 2018 Apr 11. PMID: 29637793; PMCID: PMC6233320.