Primary Information |
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BoMiProt ID | Bomi7129 |
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Protein Name | Migration and invasion enhancer 1 |
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Organism | Bos taurus |
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Uniprot ID | Q148C8 |
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Milk Fraction | Whey |
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Ref Sequence ID | NP_001068689.1 |
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Aminoacid Length | 115 |
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Molecular Weight | 12342 |
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FASTA Sequence |
Download |
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Gene Name | MIEN1 |
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Gene ID | 505710 |
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Protein Existence Status | reviewed |
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Secondary Information |
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Protein Function | involved in cytoskeletal focal adhesion dynamics.Increases cell migration by inducing filopodia formation.Also considered an oncogenic protein, because MIEN1 overexpression functionally enhances migration and invasion of tumor cells via modulating the activity of AKT. |
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Biochemical Properties | MIEN1 protein contains a consensus sequence for prenylation comprising the last 4 amino acids, CVIL, at the C terminus, a CAAX motif predicted to be post-translationally modified by protein geranylgeranyltransferase-1.It contains a central α/β core domain consisting of a mixed parallel/anti-parallel four-stranded β-sheet with two distorted α-helices. |
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PTMs | Acetylation, Disulfide bond formation, Lipidation, Prenylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| NA |
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Predicted Disorder Regions | 1-23, 99-103, 108-111 |
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DisProt Annotation | |
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TM Helix Prediction | 6TMHs; (52-74), (85-107), (130-148), (161-183), (217-239), (252-270) |
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Significance of PTMs | contains a consensus sequence for prenylation comprising the last 4 amino acids.Geranylation of MIEN1 at the CAAX motif facilitated the association of the protein to the inner leaflet of plasma membrane, enhancing the migratory phenotype of cells by inducing increased filopodia formation and potentiating directional migration.-CAAX- motif in the C terminal is predicted to be post-translationally modified by protein geranylgeranyltransferase-1. |
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Bibliography | 1.Hsu CH, Shen TL, Chang CF, Chang YY, Huang LY. Solution structure of the oncogenic MIEN1 protein reveals a thioredoxin-like fold with a redox-active motif. PLoS One. 2012;7(12):e52292. doi: 10.1371/journal.pone.0052292. Epub 2012 Dec 20. PMID: 23284973; PMCID: PMC3527542. |