Primary Information |
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| BoMiProt ID | Bomi7002 |
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| Protein Name | Meiosis regulator and mRNA stability factor 1/Limkain-b1/ |
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| Organism | Bos taurus |
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| Uniprot ID | E1BP74 |
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| Milk Fraction | Whey |
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| Aminoacid Length | 1742 |
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| Molecular Weight | 192127 |
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| FASTA Sequence |
Download |
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| Gene Name | MARF1/LKAP |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | A novel autoantigen that co-localizes with a subset of cytoplasmic microbodies. |
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| Biochemical Properties | Tandem RNA-binding domain motifs (RRM, RPN-1) at amino acids 514–580 and 784–856 are present in LKAP |
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| PTMs | Phosphorylation at Ser |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|E1BP74|MARF1_BOVIN Meiosis regulator and mRNA stability factor 1 OS=Bos taurus OX=9913 GN=MARF1 PE=3 SV=2
MEGSGTENPCSTTVGWLQQDNDAKPWLWRFSNCFSRPQQTLPLSPQTKDYMENKKVAVEL
KDVPS*65PLHAGSKLFPAVPLPDIHSLQQPKVQLSTVPKVSCCAHCTNDPSTSPVRFGGGSG
GGAGSLVPPGALLDSQSTRTITCQVGSGLAFQSAPPLQNASARNTLAGVASDFPSMCLES
NLSSCKHLPCCGKLHFQSCRGNVHKLHQFPALQGCPSSAGYFPCSDFTSGAPGHVEEHVS
QSELTPHLCTNSLHLNVVPPVCLKGSLYCEDCLSKPARNSIIDAAKVWPNIPPPNTQTAP
VTVPLCNGCGTKGMGKETTLLLATSLGKAASKFGSPEVALAGQMLENLPPIGVFWDIENC
SVPSGRSATAVVQRIREKFFKGHREAEFICVCDISKENKEVIQELNNCQVTVAHINATAK
NAADDKLRQSLRRFANTHTAPATVVLVSTDVNFALELSDLRHRHGFHIILVHKNQASEAL
LHHANELIRFEEFISDLPPRLPLKMPCHTLLYVYNLPANKDGKSISNRLRRLSDNCGGKV
LNITGCSAILRFINRDSAERALKRMENEDVFGNRIVVSFTPKSSELCETKSSNATADKVK
SPKKLKNPKLCLIKDISESPSSAKAAPGKGSQANSGSATRNANVKSLQELCRLESKTGTR
SSEPQQGHLRLGAPPHRSSSAAAPAPKAPGLAESVY*696KTNPKKESLGARSVTSSPVEKKEK
EETLFQVSYPSAFSKLIASRQVSPLLTAQPWSSRNLS*757PNLLNRASPLAFNAAHSSVGADG
PDPFAHGVDVQISNLDYRLSRKELQQLMQEAFSRHGKVKSVELSPHTDYQLKAVVQMENL
QEAIGAVNSLHRYKIGSKKILVSLATGAANKSLSLLSAETMSILQDAPACCLPLFKFTDI
YEKKFGHRLNVSDLYKLTDTVAIREQGNGRLVCLLPSSQARQSPLGSSQSHDGSSTNCSP
IIFEELEYHEPVCRQHCPNKDFSEHEFDPDSYKIPFVILSLKTFAPQVHSLLQTHEGTVP
LLSFPDCYAAEFGELEIVQENRGGGVPLEHLITCVPGVNIATAQNGVKVVKWIHNKPPPP
NTDPWLLRS*1089KS*1091PVGNPQLIQFSREVIDLLKNQPSCVIPISNFIPSYHHHFAKQCRVSDYG
YSKLIELLEAVPHVLQILGMGSKRLLTLTHRAQVKRFTQDLLKLLKSQASKQVIVKEFAQ
AYHWCFSKDWDVTEYGVCELIDIISEIPDTTICLSQQDSEAMICIPRRERTQDEIERTKQ
FSKDVVDLLRHQPHFRMPFNKFIPSYHHHFGRQCKLAYYGFTKLLELFEAIPDILQVLEC
GEEKILTLTEVERFKALAAQFVKLLRSQKDNCLMMTDLLKEYAKTFGYTFRLQDYDVSSV
SALTQKLCHVVKVADMESGKQIQLINRKSLRALTAQLLVLLMSWEGTTHLSVDELKRHYE
STHSTPLNPCEYGFMTLTELLKSLPYLVEDQVFTNDKTEECVKLTSLYLFAKNVRSLLHT
YHYQQLFLHEFSMAYSKYVGETLQPKTYGFSSVEELLGAIPQVVWIKGHGHKRIVVLKND
MKSRVNSLGPS*1571PASHETQPSAPERILEVPESPPASELRLGVGGDGPHPAEQELLRLTDDS
PVDLLCAPVPSCLPSPQLRPDPVVLQAADLIWFEEHPQEPSEIMILNQEEKIEIPVPIRN
ENLPPDPSSPGVSAAVPAPPSPSSETPESLLSKDPTESPAKKQPKNRVKLAANFSFAPIT
KL
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| Predicted Disorder Regions | 1600-1704 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | Many proteins that localize to peroxisomes contain a conserved targeting signal (MICROBODIES_CTR) in the last three amino acids in the carboxyl terminus. The LKAP carboxyl terminus sequence TKL (Thr-Lys-Leu) fits the MICROBODIES_CTR consensus sequence. |
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| Bibliography | 1.Dunster K, Lai FP, Sentry JW. Limkain b1, a novel human autoantigen localized to a subset of ABCD3 and PXF marked peroxisomes. Clin Exp Immunol. 2005 Jun;140(3):556-63. doi: 10.1111/j.1365-2249.2005.02774.x. PMID: 15932519; PMCID: PMC1809386. 2.Gould SJ, Keller GA, Schneider M et al. Peroxisomal protein
import is conserved between yeast, plants, insects and mammals.
EMBO J 1990; 9:85–90. |
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