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Primary Information | |
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| BoMiProt ID | Bomi6828 |
| Protein Name | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial/Branched-chain alpha-keto acid dehydrogenase complex component E2/ Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase/Dihydrolipoamide branched chain transacylase/Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex/BCKAD-E2/BCKADE2 |
| Organism | Bos taurus |
| Uniprot ID | P11181 |
| Milk Fraction | Whey |
| Ref Sequence ID | NP_776330.1 |
| Aminoacid Length | 482 |
| Molecular Weight | 53410 |
| FASTA Sequence | Download |
| Gene Name | DBT |
| Protein Existence Status | Reviewed |
Secondary Information | |
| Protein Function | Lipoamide acyltransferase component (E2)of The branched-chain alpha-keto dehydrogenase complex. Its function is to transfer of the acyl group from the lipoyl moiety to coenzyme A.the function of The branched-chain alpha-keto dehydrogenase complex is conversion of alpha-keto acids to acyl-CoA and CO2. |
| Biochemical Properties | The 52 000-dalton subunit is the E2 transacylase that contains the functional lipoyl moiety. |
| PTMs | Acetylation on Lys and Phosphorylation on Ser |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|P11181|ODB2_BOVIN Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Bos taurus OX=9913 GN=DBT PE=1 SV=2 MAAALVLRTWSRAAGQLICVRYFQTCGNVHVLKPKYVCFFGYPPFKYSHPYQWLKTTAAL QGQIVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKK LYYNLDDTAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGQKTLATPAVRRL AMENNIKLSEVIGSGKDGRILKEDILNYLEKQTGAILPPS*120PKAEIMPPPPKPKDRTIPIP ISKPPVFIGKDRTEPVKGFHKAMVKTMSAALKIPHFGYCDEVDLTELVKLREELKPIAFA RGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNVK NVQIRSIFEIATELNRLQKLGSAGQLSTNDLIGGTFTLSNIGSIGGTYAKPVILPPEVAI GALGTIKALPRFNEKGEVCKAQIMNVSWSADHRIIDGATVSRFSNLWKSYLENPAFMLLD LK |
| SCOP | Class : Alpha and beta proteins (a/b) Fold : CoA-dependent acyltransferases Superfamily : CoA-dependent acyltransferases Family : CAT-like Domain Name : 2IHW A:188-421 |
| CATH | Matched CATH superfamily 3.30.559.10 |
| Predicted Disorder Regions | 142-171, 213-244 |
| DisProt Annotation | |
| TM Helix Prediction | No TM helices |
| PDB ID | 2IHW,2II3,2II4,2II5, |
| Bibliography | 1.Heffelfinger SC, Sewell ET, Danner DJ. Identification of specific subunits of highly purified bovine liver branched-chain ketoacid dehydrogenase. Biochemistry. 1983 Nov 22;22(24):5519-22. doi: 10.1021/bi00293a011. PMID: 6652074. 2.Pettit FH, Yeaman SJ, Reed LJ. Purification and characterization of branched chain alpha-keto acid dehydrogenase complex of bovine kidney. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4881-5. doi: 10.1073/pnas.75.10.4881. PMID: 283398; PMCID: PMC336225. |