Search by BoMiProt ID - Bomi66

Primary Information

BoMiProt ID Bomi66
Protein Name Myosin 10
Organism Bos taurus
Uniprot IDQ27991
Milk FractionWhey, Exosome
Ref Sequence ID NP_777259.1
Aminoacid Length 1976
Molecular Weight 229099
FASTA Sequence Download
Gene Name MYH10
Gene ID 317655
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondary Information

Protein Function motor protein involved in epithelial spindle dynamics; Depletion of Myo10 results in spindle lengthening, pole fragmentation, and metaphase delay; the requirement for Myo10 in spindle pole integrity is F-actin independent; participates in essential intracellular processes such as filopodia formation/extension, phagocytosis, cell migration, and mitotic spindle maintenance
Biochemical Properties binds to Wee1, a conserved regulator of cyclin-dependent kinase 1; majority of molecules are dimeric with a head-to-head contour distance of ∼50 nm; motility assays show that myosin- 10 moves actin filaments smoothly with a velocity of ∼310 nm/s; Single molecule optical tweezers experiments show that under intermediate load (∼0.5 pN), myosin- 10 interacts intermittently with actin and produces a power stroke of ∼17 nm, composed of an initial 15-nm and subsequent 2-nm movement; ATPase activity was found to be regulated both by Ca2+ and phospholipids
Significance in milk Cytoskeletal proteins; found increased during infection
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions 1127-1149,1697-1718,1874-1976
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments Myo10 association is critical for the bacterial infection to spread; the role of Myo10 and filopodia in bacterial infection as well. Shigella flexneri is an intracellular pathogen that primarily infects mammalian epithelial cells. In order to enter eukaryotic cells, Shigella can be captured by filopodia, which then retract bringing the bacteria to the cell body
Bibliography 1. Takagi, Y., Farrow, R. E., Billington, N., Nagy, A., Batters, C., Yang, Y., … Molloy, J. E. (2014). Myosin-10 produces its power-stroke in two phases and moves processively along a single actin filament under low load. Proceedings of the National Academy of Sciences of the United States of America, 111(18), E1833-42.
2. Sandquist, J. C., Larson, M. E., Woolner, S., Ding, Z., & Bement, W. M. (2018). An interaction between myosin-10 and the cell cycle regulator Wee1 links spindle dynamics to mitotic progression in epithelia. The Journal of Cell Biology, 217(3), 849–859.