Primary Information |
|---|
| BoMiProt ID | Bomi66 |
|---|
| Protein Name | Myosin 10 |
|---|
| Organism | Bos taurus |
|---|
| Uniprot ID | Q27991 |
|---|
| Milk Fraction | Whey, Exosome |
|---|
| Ref Sequence ID | NP_777259.1 |
|---|
| Aminoacid Length | 1976 |
|---|
| Molecular Weight | 229099 |
|---|
| FASTA Sequence |
Download |
|---|
| Gene Name | MYH10 |
|---|
| Gene ID | 317655 |
|---|
| Protein Existence Status | Reviewed: Experimental evidence at transcript level |
|---|
Secondary Information |
|---|
| Protein Function | motor protein involved in epithelial spindle dynamics; Depletion of Myo10 results
in spindle lengthening, pole fragmentation, and metaphase delay; the requirement for Myo10 in spindle pole integrity is F-actin independent; participates in essential intracellular processes such as filopodia formation/extension, phagocytosis, cell migration, and mitotic spindle maintenance |
|---|
| Biochemical Properties | binds to Wee1, a conserved regulator of cyclin-dependent kinase
1; majority of molecules are dimeric with a head-to-head contour
distance of ∼50 nm; motility assays show that myosin-
10 moves actin filaments smoothly with a velocity of ∼310 nm/s; Single molecule optical tweezers
experiments show that under intermediate load (∼0.5 pN), myosin-
10 interacts intermittently with actin and produces a power
stroke of ∼17 nm, composed of an initial 15-nm and subsequent
2-nm movement; ATPase activity was found to be regulated both by Ca2+ and
phospholipids |
|---|
| Significance in milk | Cytoskeletal proteins; found increased during infection |
|---|
Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| |
|---|
| Predicted Disorder Regions | 1127-1149,1697-1718,1874-1976 |
|---|
| DisProt Annotation | |
|---|
| TM Helix Prediction | No TM helices |
|---|
| Additional Comments | Myo10 association is critical for the bacterial infection to spread; the role of Myo10 and filopodia in bacterial infection as well. Shigella flexneri is an intracellular pathogen that primarily infects mammalian epithelial cells. In order to enter eukaryotic cells, Shigella can be captured by filopodia, which then retract bringing the bacteria to the cell body |
|---|
| Bibliography | 1. Takagi, Y., Farrow, R. E., Billington, N., Nagy, A., Batters, C., Yang, Y., … Molloy, J. E. (2014). Myosin-10 produces its power-stroke in two phases and moves processively along a single actin filament under low load. Proceedings of the National Academy of Sciences of the United States of America, 111(18), E1833-42. https://doi.org/10.1073/pnas.1320122111.
2. Sandquist, J. C., Larson, M. E., Woolner, S., Ding, Z., & Bement, W. M. (2018). An interaction between myosin-10 and the cell cycle regulator Wee1 links spindle dynamics to mitotic progression in epithelia. The Journal of Cell Biology, 217(3), 849–859. https://doi.org/10.1083/jcb.201708072. |
|---|
