Primary Information |
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| BoMiProt ID | Bomi6416 |
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| Protein Name | Insulin-like growth factor-binding protein 6/IBP-6/IGF-binding protein 6/IGFBP-6 |
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| Organism | Bos taurus |
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| Uniprot ID | Q05718 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001035585.1 |
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| Aminoacid Length | 237 |
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| Molecular Weight | 24967 |
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| FASTA Sequence |
Download |
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| Gene Name | IGFBP6 |
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| Gene ID | 404186 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | inhibition of angiogenesis and promotion of cancer cell migration |
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| Biochemical Properties | share a three-domain structure.The N-domain contains a highly conserved high-affinity IGF binding subdomain that has two disulphide linkages stabilising a globular structure containing a three-stranded anti-parallel β-sheet.The N-terminal subdomains of IGFBPs 1–5 contain a conserved GCGCC motif and a ladder-like structure stabilised by four disulphide bonds.IGFBP-6 differs from other IGFBPs as it lacks this cysteine-rich motif and only has three disulphide bonds. Further, a peptide based on this subdomain is predominantly extended with minimal secondary structure.The C-domain contains three homologous disulphide linkages, a conserved CWCV sequence and a thyroglobulin type 1 fold comprising an α -helix followed by a loop, a three-stranded antiparallel β-sheet incorporating a second loop, and finally a disulphide-bonded flexible third loop. The C-domain binds IGFs through a largely hydrophobic surface involving the α-helix, the first β-strand, and the first and second loops. |
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| Significance in milk | The concentration of IGFBPs is high in bovine prepartum milk-like secretions (BPMS), colostrum and late lactation milk, and low in milk of mid lactation. |
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| PTMs | Disulfide bond formation,Glycosylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| NA |
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| Predicted Disorder Regions | 5 predicted disordered proteins; (12-15),(27-173),(178-182), (188-205), (212-237) |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | Serum levels of IGFBP-6 increase gradually with age and are higher in men than in women |
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| Bibliography | 1.Meyer Z, Höflich C, Wirthgen E, Olm S, Hammon HM, Hoeflich A. Analysis of the IGF-system in milk from farm animals - Occurrence, regulation, and biomarker potential. Growth Horm IGF Res. 2017 Aug;35:1-7. doi: 10.1016/j.ghir.2017.05.004. Epub 2017 May 12. PMID: 28544872. 2.Bach LA, Fu P, Yang Z. Insulin-like growth factor-binding protein-6 and cancer. Clin Sci (Lond). 2013 Feb;124(4):215-29. doi: 10.1042/CS20120343. PMID: 23126425. |
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