Search by BoMiProt ID - Bomi6416

Primary Information

BoMiProt ID Bomi6416
Protein Name Insulin-like growth factor-binding protein 6/IBP-6/IGF-binding protein 6/IGFBP-6
Organism Bos taurus
Uniprot IDQ05718
Milk FractionWhey
Ref Sequence ID NP_001035585.1
Aminoacid Length 237
Molecular Weight 24967
FASTA Sequence Download
Gene Name IGFBP6
Gene ID 404186
Protein Existence Status reviewed

Secondary Information

Protein Function inhibition of angiogenesis and promotion of cancer cell migration
Biochemical Properties share a three-domain structure.The N-domain contains a highly conserved high-affinity IGF binding subdomain that has two disulphide linkages stabilising a globular structure containing a three-stranded anti-parallel β-sheet.The N-terminal subdomains of IGFBPs 1–5 contain a conserved GCGCC motif and a ladder-like structure stabilised by four disulphide bonds.IGFBP-6 differs from other IGFBPs as it lacks this cysteine-rich motif and only has three disulphide bonds. Further, a peptide based on this subdomain is predominantly extended with minimal secondary structure.The C-domain contains three homologous disulphide linkages, a conserved CWCV sequence and a thyroglobulin type 1 fold comprising an α -helix followed by a loop, a three-stranded antiparallel β-sheet incorporating a second loop, and finally a disulphide-bonded flexible third loop.  The C-domain binds IGFs through a largely hydrophobic surface involving the α-helix, the first β-strand, and the first and second loops.
Significance in milk The concentration of IGFBPs is high in bovine prepartum milk-like secretions (BPMS), colostrum and late lactation milk, and low in milk of mid lactation.
PTMs Disulfide bond formation,Glycosylation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions 5 predicted disordered proteins; (12-15),(27-173),(178-182), (188-205), (212-237)
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments Serum levels of IGFBP-6 increase gradually with age and are higher in men than in women
Bibliography 1.Meyer Z, Höflich C, Wirthgen E, Olm S, Hammon HM, Hoeflich A. Analysis of the IGF-system in milk from farm animals - Occurrence, regulation, and biomarker potential. Growth Horm IGF Res. 2017 Aug;35:1-7. doi: 10.1016/j.ghir.2017.05.004. Epub 2017 May 12. PMID: 28544872. 2.Bach LA, Fu P, Yang Z. Insulin-like growth factor-binding protein-6 and cancer. Clin Sci (Lond). 2013 Feb;124(4):215-29. doi: 10.1042/CS20120343. PMID: 23126425.