Primary Information |
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| BoMiProt ID | Bomi6409 |
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| Protein Name | Inositol-tetrakisphosphate 1-kinase / Inositol 1,3,4-trisphosphate 5/6-kinase |
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| Organism | Bos taurus |
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| Uniprot ID | P0C0T1 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001179418.1 |
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| Aminoacid Length | 419 |
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| Molecular Weight | 45842 |
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| FASTA Sequence |
Download |
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| Gene Name | ITPK1 |
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| Gene ID | 518488 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | ITPK1 is the rate limiting enzyme in the pathway leading to formation of the highly phosphorylated inositol phosphates including IP6 and the inositol pyrophosphates.It is a kinase that phosphorylates inositol polyphosphates such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. It forms Ins(1,3,4,5,6)P5 from Ins(3,4,5,6)P4 by phosphorylation at position 1. |
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| Biochemical Properties | 1D-myo-inositol 3,4,5,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H+.Binds 2 magnesium ions per subunit as cofactor.KM=80 nM for Ins(1,3,4)P3 and Vmax=60 nmol/min/mg enzyme with Ins(1,3,4)P3 as substrate. |
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| PTMs | Acetylation and Phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|P0C0T1|ITPK1_BOVIN Inositol-tetrakisphosphate 1-kinase OS=Bos taurus OX=9913 GN=ITPK1 PE=1 SV=1
MQTFLKGKRVGYWLSEKKIKKLNFQAFAELCRKRGIEVVQLNLSRPIEEQGPLDVIIHKL
TDVILEADQNDSQALELVHRFQEYIDAHPETIVLDPLPAIRTLLDRSKSYELIRKIEAYM
KDDRICSPPFMELTSLCGDDTMRLLEENGLAFPFICKTRVAHGTNSHEMAIVFNQEGLSA
IQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPES
SSVLTALDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFP
GYEGVSEFFTDLLNHIASVLQGQSSGVAGAGDVAPLKHSRLLAEQAGGLAAERTCSASPG
CCSSMMGQEPPWTPEADMGGVGAGSTAKLPHQRLGCTAGVS*401PSFQQHCVASLATKASSQ
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| Predicted Disorder Regions | (330-334), (358-391), (414-419) |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | Acetylation by EP300 and CREBBP destabilizes ITPK1, and down-regulates enzymatic activity. Deacetylated by SIRT1 |
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| Bibliography | Wilson, M. P., & Majerus, P. W. (1996). Isolation of inositol 1,3,4-trisphosphate 5/6-kinase, cDNA cloning and expression of the recombinant enzyme. The Journal of biological chemistry, 271(20), 11904–11910. https://doi.org/10.1074/jbc.271.20.11904 |
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