Primary Information |
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| BoMiProt ID | Bomi6322 |
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| Protein Name | Hormone-sensitive lipase/HSL/Monoacylglycerol lipase LIPE/Retinyl ester hydrolase/REH |
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| Organism | Bos taurus |
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| Uniprot ID | P16386 |
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| Milk Fraction | Whey,MFGM |
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| Ref Sequence ID | NP_001073689.1 |
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| Aminoacid Length | 756 |
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| Molecular Weight | 82641 |
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| FASTA Sequence |
Download |
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| Gene Name | LIPE |
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| Gene ID | 286879 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | hydrlysis of triacylglycerols and diacylglcerols. |
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| Biochemical Properties | molecular mass of approx. 84 kDa, comprises three major domains: a catalytic domain, a regulatory domain encoding several phosphorylation sites and an N-terminal domain involved in protein-protein and protein-lipid interactions. |
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| Significance in milk | Essential role in lipomobilization,for dairy cows to balance the energy requirement for milk production in early lactation. |
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| PTMs | phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|P16386|LIPS_BOVIN Hormone-sensitive lipase OS=Bos taurus OX=9913 GN=LIPE PE=1 SV=2
MDLRTMTQSLVTLAEDNMAFFSSQGPGETARRLTGVFAGIREQALGLEPALGRLLSVAHL
FDLDTETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQ
LRALAYYAQRLLTTNQPGRLFFEGDERVIADFLREYVTLHKGCFYGRCLGFQFTPAIRPF
LQTISIGLVSFGEHYKRNETGISVTASSLFTDGRFAIDPELRGAEFERIIQNLDVHFWKA
FWNITEIQVLSSLANMASTTVRVSRLLSLPPVAFEMPLTSDPELTVTISPPLAHTGPGPV
LVRLISYDLREGQDSKELSSFVRSEGPRSLELRLRPQQAPRSRALVVHIHGGGFVAQTSK
SHEPYLKSWAQELGAPILSIDYSLAPEAPFPRALEECFYAYCWAVKHCALLGSTGERICL
AGDSAGGNLCFTVSLRAAAYGVRVPDGIMAAYPATMLQSTASPSRLLSLMDHLLPLSVLS
KCVSTYAGAETEDHPDSDQKALGVMGLVQRDTALLLRDLRLGASSWLNSFLELGGQKSHL
KSVSKTEPMRRS*552VS*554EAALTQPEGSLGTDSLKSLKLHDLGLRNSSDTTDTPELSLS*595AETLGPSAPSTINFLLGSEDGSEMSKAPEELNNKDRVRGVGAAFPEGFHPRRCS*649QGAMWMPLYSA
PIVKNPSMSPLLAPDSMLQTLPPVHIVACALDPMLDDSVMFARRLRGLGQPVTLRVAEDL
PHGFLSLAALCRETRQAAALCVERIRLILNLPGPPV
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | no TM helices |
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| Significance of PTMs | Reversible phosphorylation mediates its activation by lipolytic hormones. phosphorylated at two sites within the polypeptide, with one site(Ser-565) being phosphorylated under basal conditions, and a second site(Ser 563) being phosphorylated under lipolytic stimulation by cAMP-dependent protein kinase.The basal site has the potential to block modification of the regulatory site and thus exert an anti-lipolytic effect on the enzyme. |
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| Bibliography | 1.Locher LF, Meyer N, Weber EM, Rehage J, Meyer U, Dänicke S, Huber K. Hormone-sensitive lipase protein expression and extent of phosphorylation in subcutaneous and retroperitoneal adipose tissues in the periparturient dairy cow. J Dairy Sci. 2011 Sep;94(9):4514-23. doi: 10.3168/jds.2011-4145. PMID: 21854923. 2.Yeaman SJ. Hormone-sensitive lipase--new roles for an old enzyme. Biochem J. 2004 Apr 1;379(Pt 1):11-22. doi: 10.1042/BJ20031811. PMID: 14725507; PMCID: PMC1224062. |
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