Search by BoMiProt ID - Bomi6287

Primary Information

BoMiProt ID Bomi6287
Protein Name Histone H2A.J
Organism Bos taurus
Uniprot IDQ3ZBX9
Milk FractionWhey
Ref Sequence ID NP_001071557.1
Aminoacid Length 129
Molecular Weight 14019
FASTA Sequence Download
Gene Name H2AJ
Gene ID 618489
Protein Existence Status Reviewed

Secondary Information

Protein Function H2A.J promotes inflammatory gene expression in senescence.required for the production of SASP (senescent-associated secretory proteins)factors.
Biochemical Properties The specific C-terminus of H2A.J is important for its function in promoting inflammatory gene expression in senescence.H2A.J differs from canonical H2A by only five amino acids involving a substitution of Val for Ala at position 11 in the N-terminal tail, and a unique C terminus containing a potentially phosphorylatable SQ motif.Conserved Val11 and Ser-123 are functionally important for H2A.J transcriptional activity.
PTMs Acetylation, Isopeptide bond formation, Methylation, Phosphorylation, Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions 1-33, 114-129
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs phosphorylation of H2A.J on Ser-123 which is important for DNA-nucleosome interaction.Phosphorylation of the SQ motif plays a role in DNA-damage responses.Monoubiquitination of Lys-120 gives a specific tag for epigenetic transcriptional repression.Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties.Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation at Thr-121 (H2AT120ph) by DCAF1 is present in the regulatory region of many tumor suppresor genes and down-regulates their transcription
Bibliography 1.Contrepois, K., Coudereau, C., Benayoun, B. et al. Histone variant H2A.J accumulates in senescent cells and promotes inflammatory gene expression. Nat Commun 8, 14995 (2017). 2.Foster ER, Downs JA. Histone H2A phosphorylation in DNA double-strand break repair. FEBS J. 2005 Jul;272(13):3231-40. doi: 10.1111/j.1742-4658.2005.04741.x. PMID: 15978030.