Primary Information |
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| BoMiProt ID | Bomi6253 |
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| Protein Name | Hexokinase-1/Brain form hexokinase/Hexokinase type I/HK I |
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| Organism | Bos taurus |
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| Uniprot ID | P27595 |
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| Milk Fraction | Whey |
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| Aminoacid Length | 918 |
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| Molecular Weight | 103064 |
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| FASTA Sequence |
Download |
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| Gene Name | HK1 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Catalyzes the phosphorylation of hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate. |
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| Biochemical Properties | In mammals there are four HK isozymes, which vary in their tissue distribution and kinetic properties. Each of the types l-3 HK isozymes consists of a single polypeptide chain with a molecular weight of approximately 100 kDa and is inhibited by the product glucose 6-phosphate.Putative ATP- and glucose-binding domains identified in the carboxy-terminal (catalytic) half of bovine Hexokinase.Ser-178 is positioned within the putative core of glucose binding domain which has been found to be catalytically important for binding to glucose.This serine is involved in the formation of hydrogen bond to the 6-(OH) group of glucose. |
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| PTMs | phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|P27595|HXK1_BOVIN Hexokinase-1 OS=Bos taurus OX=9913 GN=HK1 PE=2 SV=1
MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLLDIMNRFKKEMKNGLSRDFNPTAT
VKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEQNRPVHMESEVYDTPENIMH
GSGSQLFDHVLECLGDFMEKKKIKDKKLPVGFTFSFPCRQSKIDQAILITWTKRFKARGA
EGNYVVKLLDKAIKKRGDYDANIVAVVNDTVGTMIDCGYDDQHCEVGLIIGTGTNACYME
ELRQIDFGWGDDGRMCINTEWGDLGDDGSLEDIRKEFDREFRRGSLNPGKQRFEKMVSGR
YMEDVVRLVLVKMAKEGLLFEGRITPELLTRGKFNTS*337DVSAIEKDKEGLHNAKEILTRLG
VERSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKSTPRLRTTVRVDGSLYKTH
PQYSRRFHKTLRRLVPDSDVRFLLSESGTGKGAAMVTAVAYRLAEQHRQIEETLAHFRLS
KQTLMEVKKRLRTEMEMGLRKETNSNATVNMLPSFLRSIPDGTEDGDFLALDLGGTNFRV
LLVKIRSGKKSTVEMHNKIYRIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGF
TFSFPCQQTSLDAGILITWTKGFKATDCVGHDVVTLLRDAVKRREEFDLDVVAVVNDTVG
TMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVEMVEGNQRQMCINMEWGAFGDNGCSDD
IRTDFDKVVDEYSLNSGNQRFENMISGIYLGEIVRNILIDFTKKGFLFRGQISEPLKTRG
IFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAA
VVEKIRENRGLDRLNVTVGVDGTLYKLHPQFSRIMHQTVKELSPKCNVSFLLSEDGSGKG
AALITAVGVRLRGESAIS |
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | Akt has a direct effect at the level of the mitochondrion, which is mediated via phosphorylation of HK-II and results in protection of mitochondria against oxidant or Ca2+-stimulated PT-pore opening. Akt was reported to directly phosphorylate HK2 at T473 and promote mitochondrial translocation of HK2 in cardiomyocytes. it has been reported that Akt similarly phosphorylates HK2 in cancer cells to enhance the efficiency of HK2 catalytic reaction and subsequently promotes glycolysis.Serine phosphorylation on Ser 23 position is important for enzyme localization. |
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| Bibliography | 1.Miyamoto S, Murphy AN, Brown JH. Akt mediates mitochondrial protection in cardiomyocytes through phosphorylation of mitochondrial hexokinase-II. Cell Death Differ. 2008 Mar;15(3):521-9. doi: 10.1038/sj.cdd.4402285. Epub 2007 Dec 7. PMID: 18064042. 2.Griffin LD, Gelb BD, Wheeler DA, Davison D, Adams V, McCabe ER. Mammalian hexokinase 1: evolutionary conservation and structure to function analysis. Genomics. 1991 Dec;11(4):1014-24. doi: 10.1016/0888-7543(91)90027-c. PMID: 1783373. |
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