Primary Information | |
---|---|
BoMiProt ID | Bomi6177 |
Protein Name | Guanylate cyclase soluble subunit alpha-1(GCS-alpha-1)/Soluble guanylate cyclase large subunit |
Organism | Bos taurus |
Uniprot ID | P19687 |
Milk Fraction | Whey |
Ref Sequence ID | NP_786972.1 |
Aminoacid Length | 691 |
Molecular Weight | 77533 |
FASTA Sequence | Download |
Gene Name | GUCY1A1/GUC1A1 |
Gene ID | 281216 |
Protein Existence Status | reviewed |
Secondary Information | |
Protein Function | soluble guanylyl cyclase (sGC) is a heterodimeric enzyme with an α and β subunit.role in suppression of VSMC(vascular smooth muscle cells) proliferation and vasorelaxation. |
Biochemical Properties | alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme.catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate.Responds to Nitric oxide which is a relaxant neurotransmitter.Soluble GC contains a C-terminal catalytic domain, a central dimerization domain, and an N-terminal regulatory domain. existence of an amphipathic alpha-helix in residues 431-464 in the alpha subunit. amphipathicity of the alpha-helix is important for the formation of the heterodimer, and Leu(463) in the alpha-helix region plays a critical role in the formation of a properly arranged active center in the dimer. |
PTMs | phosphorylation on Ser |
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | NA |
CATH | Matched CATH superfamily n/a |
Predicted Disorder Regions | 23-65, 686-691 |
DisProt Annotation | |
TM Helix Prediction | No TM helices |
Significance of PTMs | phosphorylation of α1-subunit by protein kinase A enhances the NO-dependent sGC activity, most likely by stabilizing the NO/α1β1 complex. Serine-107A and Serine 108A are the predicted site for phosphorylation. |
PDB ID | 1AWN, |
Bibliography | 1.Shiga T, Suzuki N. Amphipathic alpha-helix mediates the heterodimerization of soluble guanylyl cyclase. Zoolog Sci. 2005 Jul;22(7):735-42. doi: 10.2108/zsj.22.735. PMID: 16082162.2.Korkmaz Y, Roggendorf HC, Siefer OG, Seehawer J, Imhof T, Plomann M, Bloch W, Friebe A, Huebbers CU. Downregulation of the α1- and β1-subunit of sGC in Arterial Smooth Muscle Cells of OPSCC Is HPV-Independent. J Dent Res. 2018 Oct;97(11):1214-1221. doi: 10.1177/0022034518774531. Epub 2018 May 18. PMID: 29775416. 3.Kostic TS, Andric SA, Stojilkovic SS. Receptor-controlled phosphorylation of alpha 1 soluble guanylyl cyclase enhances nitric oxide-dependent cyclic guanosine 5'-monophosphate production in pituitary cells. Mol Endocrinol. 2004 Feb;18(2):458-70. doi: 10.1210/me.2003-0015. Epub 2003 Nov 20. PMID: 14630997. |