Primary Information |
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| BoMiProt ID | Bomi6168 |
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| Protein Name | Guanine nucleotide exchange factor for Rab-3A/Rab-3A-interacting-like protein 1/Rab3A-interacting-like protein 1/Rabin3-like 1 |
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| Organism | Bos taurus |
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| Uniprot ID | Q2KJ58 |
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| Milk Fraction | Whey,MFGM |
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| Ref Sequence ID | NP_001039997.1 |
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| Aminoacid Length | 390 |
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| Molecular Weight | 43325 |
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| FASTA Sequence |
Download |
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| Gene Name | RAB3IL1 |
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| Gene ID | 614335 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | interacts with both InsP6K1 and Rab3A, a Ras-like GTPase that regulates synaptic vesicle exocytosis.GRAB regulates depolarization-induced adrenal chromaffin cells is indicated by augmentation
release of dopamine from PC12 cells and nicotinic of such release in cells depleted of endogenous GRAB
agonist-induced hGH release from bovine adrenal and inhibition of such release in cells transfected with
chromaffin cells. |
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| Biochemical Properties | GRAB is a physiologic GEF for Rab3A, as depletion of endogenous GRAB markedly reduces GTP loading of Rab3A. Binding of GRAB to Rab3A is mediated by a coiled-coil domain of GRAB, which also mediates its binding to InsP6K1 so that InsP6K1 and Rab3A compete for binding to GRAB |
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| PTMs | Phosphorylation at Ser |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q2KJ58|R3GEF_BOVIN Guanine nucleotide exchange factor for Rab-3A OS=Bos taurus OX=9913 GN=RAB3IL1 PE=2 SV=1
MWSGQPHPDEGHPPPLEAVPVPWKSVGPCKSHRESLGGLPETPAGEEAQGEEGPAATQLD
VSRLRSSSMEIREKGSEFLKEELHKAQKELKLKDEECERLSKVREQLEQELEELTASLFE
EAHKMVREANMKQAASEKQLKEARGKIDMLQAEVTALKTLVITSTPAS*168PNRELHPQLLS*179P
TKAGPRKGHLRHKSTSSALCPAVCPVAGHILTPDKEGKEVDTTLFAEFQAWRESPTLDKT
SPFLERVYREDVGPCLDFTMQELSALVRAAVEDNTLTIEPVASQTLPAVKVAAVDCGHTN
GFRAPIDTTCALSGLACACRHRIRLGDSESHYYISPSSRARITAVCNFFTYIRYIQQGLV
RQDAEPMFWEITRLRKEMSLAKLGFFPHEA |
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| Predicted Disorder Regions | 1-103, 114-143, 164-203 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | GRAB appears to be the mammalian ortholog of the yeast GEF Sec2p, as Sec2p is the yeast protein displaying greatest homology to GRAB, and both proteins have GEF functions |
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| Bibliography | 1.Hongbo R. Luo; Adolfo Saiardi; Eiichiro Nagata; Keqiang Ye; Hongbo Yu; Thomas S. Jung; Xiaojiang Luo; Sima Jain; Akira Sawa; Solomon H. Snyder (2001). GRAB: A Physiologic Guanine Nucleotide Exchange Factor for Rab3a, which Interacts with Inositol Hexakisphosphate Kinase. , 31(3), 0–451. doi:10.1016/s0896-6273(01)00384-1 |
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