Primary Information |
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| BoMiProt ID | Bomi6038 |
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| Protein Name | Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2/D-fructose-6-phosphate amidotransferase 2/Glutamine:fructose-6-phosphate amidotransferase 2 |
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| Organism | Bos taurus |
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| Uniprot ID | Q08DQ2 |
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| Milk Fraction | Exosomes |
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| Ref Sequence ID | NP_001070351.1 |
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| Aminoacid Length | 682 |
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| Molecular Weight | 77081 |
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| FASTA Sequence |
Download |
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| Gene Name | GFPT2 |
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| Gene ID | 530101 |
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| Protein Existence Status | Reviewed |
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Secondary Information |
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| Presence in other biological fluids/tissue/cells | diaphragm |
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| Protein Function | hGFAT2 is able to isomerize fructose-6-phosphate into glucose-6-phosphate even in the presence of equimolar amounts of glutamine, which results in unproductive glutamine hydrolysis. |
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| Biochemical Properties | The structure of GFAT is characterized by having two domains, glutaminase (GLN) and isomerase (ISOM), responsible for its enzymatic activity.Presence of a partially structured loop in the glutaminase domain, whose sequence is present in eukaryotic enzymes but absent in the E. coli homolog. Molecular dynamics simulations suggest that this loop is the most flexible portion of the protein and plays a key role on conformational states of hGFAT2. |
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| PTMs | Phosphorylation at Ser |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q08DQ2|GFPT2_BOVIN Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 OS=Bos taurus OX=9913 GN=GFPT2 PE=2 SV=1
MCGIFAYMNYRVPRTRKEIFETLIKGLQRLEYRGYDSAGVAIDGNNNEVKERHIQLVKKR
GNVKALDEELYKQDSMDLKVEFETHFGIAHTRWATHGVPSAVNSHPQRSDKGNEFVVIHN
GIITNYKDLRKFLESKGYEFESETDTETIAKLIKYVFDNRETEDITFSTLVERVIQQLEG
AFALVFKSIHYPGEAVATRRGSPLLIGVRSKYKLSTEQIPVLYRTRNIENVKNICKTRMK
RLDS*244STCLHAVGNKAVEFFFASDASAIIEHTNRVIFLEDDDIAAVADGKLSIHRVKRLAS
DDPSRAIQTLQMELQQIMKGNFSAFMQKEIFEQPESVFNTMRGRVNFETNTVLLGGLKDH
LKEIRRCRRLIVIGCGTSYHAAVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFF
ISQSGETADTLLALRYCKDRRALTVGVTNTVGSSISRETDCGVHINAGPEIGVASTKAYT
SQFISLVMFGLMMSEDRISLQNRRREIIHGLKSLPELIKEVLSLDEKIHDLALELYTQRS
LLVMGRGYNYATCLEGALKIKEITYMHSEGILAGELKHGPLALIDKQMPVIMVIMKDPCF
AKCQNALQQVTARQGRPIILCSKDDTESSKFAYKTIELPHTVDCLQGILSVIPLQLLSFH
LAVLRGYDVDFPRNLAKSVTVE |
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | Overexpression contributes to insulin resistance and higher O-GlcNAc levels. |
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| Bibliography | 1.Oliveira IA, Allonso D, Fernandes TVA, Lucena DMS, Ventura GT, Dias WB, Mohana-Borges RS, Pascutti PG, Todeschini AR. Enzymatic and structural properties of human glutamine:fructose-6-phosphate amidotransferase 2 (hGFAT2). J Biol Chem. 2021 Jan-Jun;296:100180. doi: 10.1074/jbc.RA120.015189. Epub 2020 Dec 17. PMID: 33303629; PMCID: PMC7948480. |
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