Primary Information |
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| BoMiProt ID | Bomi5616 |
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| Protein Name | Eukaryotic translation initiation factor 2D/Ligatin |
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| Organism | Bos taurus |
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| Uniprot ID | Q58CR3 |
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| Milk Fraction | Whey,MFGM |
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| Ref Sequence ID | NP_001030276.1 |
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| Aminoacid Length | 579 |
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| Molecular Weight | 63647 |
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| FASTA Sequence |
Download |
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| Gene Name | EIF2D/LGTN |
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| Gene ID | 511844 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | role in translation re-initiation and ribosome recycling.eIF2D interacts with the 40S ribosomal subunit and initiator tRNA to promote translation initiation . |
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| Biochemical Properties | has eIF1-like domain, crucial for scanning and the fidelity of AUG recognition.contains SWIB/MDM2, linker and SUI1 domains. The SUI1 domain is connected to the linker by a small loop 485KGRICP490. Though the G486 residue in the loop could possibly impart flexibility to the orientation of the SUI1 domain with respect to the rest of the protein, extensive electrostatic and hydrophobic interactions between the SUI1 domain and the linker confers rigidity to the entire structure .SUI1 domain would anchor eIF2D on the ribosome, while the SWIB/MDM2 domain might prevent the simultaneous binding of eIF2 to the ribosome. |
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| PTMs | Acetylation and phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q58CR3|EIF2D_BOVIN Eukaryotic translation initiation factor 2D OS=Bos taurus OX=9913 GN=EIF2D PE=2 SV=1
MFAKAFRVKSNTAIKGSDRRKLRADVAAVFPTLGTDQVSELVPGKEELNIVKLYAHRGDA
VTVYVSGGNPILFELEKNLYPTVYTLWSYPDLLPTFTTWPLVLEKLVGGADLMLPGLVVP
PAGLPQVQKGDLCAVALVGNRAPVAVGVAAMSTAEMLASGLKGRGFCVLHSYQDHLWRSG
DKSSPPSIAPLALNPPDLSEGKGCVKADTALQGAMRQLTLEEEVQQRCEEKS*232PSEATEDP
GPGGLHVDPMDSKTLQEQMDELLQTCFLHALKCSVRKADLPLLTSTLLGSHMFSCCPEGR
QLDIKKSSYKKLSKFLQHMQQEQIIQVQELSKGVESIVAVDWKHPRITSFVIPEPS*356PTSQ
TIQEGSREQPYHPPDIKPLYCVPASMTLLFQESGHKKGSVLEGSEVRTFVINYAKKNDLV
DADNKNLVKLDPILCDCILEKDEQHTVTKLPWDSLLGRCLEKLQPAYQVTFPGQEPIVKK
GRICPIDITLAQKASNKKVTVVRNLEAYGLDPRSVAATLQQRCQASTTVTSAPGLKDSVQ
VQIQGNQIHHLGRLLLEEYRLPRKHIQGLEKAPKPGKKK |
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| Predicted Disorder Regions | 180-197, 221-251, 306-312, 354-371, 568-579 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Bibliography | Vaidya AT, Lomakin IB, Joseph NN, Dmitriev SE, Steitz TA. Crystal Structure of the C-terminal Domain of Human eIF2D and Its Implications on Eukaryotic Translation Initiation. J Mol Biol. 2017 Sep 1;429(18):2765-2771. doi: 10.1016/j.jmb.2017.07.015. Epub 2017 Jul 21. PMID: 28736176; PMCID: PMC5572308. |
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