Search by BoMiProt ID - Bomi5510


Primary Information

BoMiProt ID Bomi5510
Protein Name Engulfment and cell motility protein 2
Organism Bos taurus
Uniprot IDA4FUD6
Milk FractionWhey
Ref Sequence ID NP_001076860.1
Aminoacid Length 720
Molecular Weight 82589
FASTA Sequence Download
Gene Name ELMO2
Gene ID 508361
Protein Existence Status reviewed

Secondary Information

Protein Function regulator of insulin-dependent Glut4 membrane translocation through modulating Rac1 activity and Akt membrane compartmentalization.interaction of Elmo1/2 with BAI (brain-specific angiogenesis inhibitor) recruits them onto the proximal membrane to activate the small GTPase Rac1 through the Dock180 guanine nucleotide exchange factor to mediate engulfment of apoptotic cells or to promote muscle differentiation.
Biochemical Properties contains Ras GTPase-binding domain,a pleckstrin homology(PH) domain and a proline-rich region .Interacts directly with the SH3-domain of DOCK1 via its SH3-binding site
PTMs phosphorylation on Ser and Tyr
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|A4FUD6|ELMO2_BOVIN Engulfment and cell motility protein 2 OS=Bos taurus OX=9913 GN=ELMO2 PE=2 SV=1 MPPPSDIVKVAIEWPGANAQLLEIDQKRPLASIIKEVCDGWSLPNPEY*48YTLRYADGPQLY ITEQTRSDIKNGTILQLAISPSRAARQLMERTQSSSMETRLDAMKELAKLSADVTFATEF INMDGIVVLTRLVESGTKLLSHYSEMLAFTLTAFLELMDHGIVSWDMVSITFIKQIAGYV SQPMVDVSILQRSLAILESMVLNSQSLYQKIAEEITVGQLISHLQVSNQEIQTYAIALIN ALFLKAPEDKRQDMANAFAQKHLRSIILNHVIRGNRPIKTEMAHQLYVLQVLTFNLLEER MMTKMDPNDQAQRDIIFELRRIAFDADSDPSNAPGSGTEKRKAMYTKDYKMLGFTNHINP AMDFTQTPPGMLALDNMLYLAKVHQDTYIRIVLENSSREDKHECPFGRSAIELTKMLCEI LQVGELPNEGRNDYHPMFFTHDRAFEELFGICIQLLNKTWKEMRATAEDFNKVMQVVREQ ITRALPSKPNSLDQFKSKLRSLS*503YSEILRLRQSERMSQDDFQSPPIVELREKIQPEILEL IKQQRLNRLCEGSSFRKIGNRRRQERFWYCRLALNHKVLHYGDLDDNPQGEVTFESLQEK IPVADIKAIVTGKDCPHMKEKSALKQNKEVLELAFSILYDPDETLNFIAPNKYEYCIWID GLSALLGKDMSSELTKSDRDTLLSMEMKLRLLDLENIQIPEAPPPVPKEPSSYDFVY*717HYG
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs Insulin promotes tyrosine phosphorylation. Axl phosphorylates Elmo1/2 on a conserved carboxyl-terminal tyrosine residue. Upon Gas6-dependent activation of Axl, endogenous Elmo2 becomes phosphorylated on Tyr-713 and enters into a physical complex with Axl in breast cancer cells,which is required for cell invasion and cell migration. tyrosine-phosphorylated residue Y713 in Elmo2 becomes a docking site for other signaling molecules,which can increase Rac binding and GEF activity .
Bibliography 1.Abu-Thuraia A, Gauthier R, Chidiac R, Fukui Y, Screaton RA, Gratton JP, Côté JF. Axl phosphorylates Elmo scaffold proteins to promote Rac activation and cell invasion. Mol Cell Biol. 2015 Jan;35(1):76-87. doi: 10.1128/MCB.00764-14. Epub 2014 Oct 20. PMID: 25332238; PMCID: PMC4295393. 2.Sun Y, Côté JF, Du K. Elmo2 Is a Regulator of Insulin-dependent Glut4 Membrane Translocation. J Biol Chem. 2016 Jul 29;291(31):16150-61. doi: 10.1074/jbc.M116.731521. Epub 2016 May 20. PMID: 27226625; PMCID: PMC4965564.