Search by BoMiProt ID - Bomi5496

Primary Information

BoMiProt ID Bomi5496
Protein Name Endoplasmic reticulum chaperone BiP /78 kDa glucose-regulated protein/Binding-immunoglobulin protein/Heat shock protein 70 family protein 5/Heat shock protein family A member 5/Immunoglobulin heavy chain-binding protein
Organism Bos taurus
Uniprot ID Q0VCX2
Milk FractionWhey
Aminoacid Length 655
Molecular Weight 72400
FASTA Sequence Download
Gene Name HSPA5/GRP78
Gene ID 415113
Protein Existence Status reviewed

Secondary Information

Protein Function protein folding and quality control in the ER lumen.correct folding of proteins and degradation of misfolded proteins.key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR).BiP performs its functions with the assistance of ER-localized DnaJ cofactors (ERdjs), which stimulate BiP's ATP-dependent substrate interactions.
Biochemical Properties Monomer and homooligomer.a highly conserved N-terminal nucleotide-binding domain (NBD), a substrate-binding domain (SBD) composed of eight β-strands with a helical lid, and a linker between them that controls the allosteric interaction between these two domains.
Significance in milk assist in the refolding of proteins during periparturient phase in which the dairy cows face with ER stress.
PTMs Acetylation, Isopeptide bond, Methylation, Nitration, Phosphoprotein, Ubl conjugation
Predicted Disorder Regions 2 predicted disordered segments;(583-586), 614th residue, (639-655)
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs AMPylation at Thr-519 by FICD inactivates the chaperone activity.interdomain linker regulates the chaperone activity.
Bibliography 1.Gessner DK, Schlegel G, Ringseis R, Schwarz FJ, Eder K. Up-regulation of endoplasmic reticulum stress induced genes of the unfolded protein response in the liver of periparturient dairy cows. BMC Vet Res. 2014 Feb 20;10:46. doi: 10.1186/1746-6148-10-46. PMID: 24555446; PMCID: PMC3936700. 2.Pobre KFR, Poet GJ, Hendershot LM. The endoplasmic reticulum (ER) chaperone BiP is a master regulator of ER functions: Getting by with a little help from ERdj friends. J Biol Chem. 2019 Feb 8;294(6):2098-2108. doi: 10.1074/jbc.REV118.002804. Epub 2018 Dec 18. PMID: 30563838; PMCID: PMC6369273.