Primary Information |
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| BoMiProt ID | Bomi5486 |
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| Protein Name | Endonuclease G, mitochondrial/Endo G |
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| Organism | Bos taurus |
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| Uniprot ID | P38447 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_787017.1 |
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| Aminoacid Length | 299 |
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| Molecular Weight | 32262 |
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| FASTA Sequence |
Download |
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| Gene Name | ENDOG |
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| Gene ID | 327707 |
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| Protein Existence Status | Reviewed |
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Secondary Information |
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| Protein Function | Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. |
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| PTMs | Disulphide bond, Phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|P38447|NUCG_BOVIN Endonuclease G, mitochondrial OS=Bos taurus OX=9913 GN=ENDOG PE=1 SV=1
MQLLRAGLTLALGAGLGAAAESWWRQRADARATPGLLSRLPVLPVAAAAGLPAVPGAPAG
GGPGELAKYGLPGVAQLKSRASYVLCYDPRTRGALWVVEQLRPEGLRGDGNRSSCDFHED
DSVHAYHRAT*130NADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNN
LEKYSRSLTRTYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAA
GGQIELRSYVMPNAPVDEAIPLEHFLVPIESIERASGLLFVPNILARAGS*290LKAITAGSK |
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | Glycogen synthase kinase 3 beta-mediated phosphorylation of ENDOG at Thr-128 and Ser-288 enhances its interaction with 14-3-3γ, which leads to the release of Tuberin (TSC2) and Phosphatidylinositol 3-kinase catalytic subunit type 3 (Vps34) from 14-3-3γ, followed by mTOR pathway suppression and autophagy initiation. |
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| Bibliography | 1.Wang, W., Li, J., Tan, J. et al. Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. Nat Commun 12, 476 (2021). https://doi.org/10.1038/s41467-020-20780-2 2.Côté, J., & Ruiz-Carrillo, A. (1993). Primers for mitochondrial DNA replication generated by endonuclease G. Science (New York, N.Y.), 261(5122), 765–769. https://doi.org/10.1126/science.7688144 |
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