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Primary Information | |
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| BoMiProt ID | Bomi5456 |
| Protein Name | Elongation factor Tu, mitochondrial/EF-Tu |
| Organism | Bos taurus |
| Uniprot ID | P49410 |
| Milk Fraction | Whey |
| Ref Sequence ID | NP_776632.1 |
| Aminoacid Length | 452 |
| Molecular Weight | 49398 |
| FASTA Sequence | Download |
| Gene Name | TUFM |
| Gene ID | 281556 |
| Protein Existence Status | reviewed |
Secondary Information | |
| Protein Function | Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays also a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIG-I/DDX58-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy. |
| Biochemical Properties | Interacts with NLRX1.Interacts with ATG16L1. Switch I of EF-Tu rapidly converts from an α-helix into a β-hairpin and moves to interact with the acceptor stem of the aa-tRNA. |
| PTMs | ADP Ribosylation,Acetylation on Lys and phosphorylation on Ser and Thr |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|P49410|EFTU_BOVIN Elongation factor Tu, mitochondrial OS=Bos taurus OX=9913 GN=TUFM PE=1 SV=1 MAAATLLRATPLFSGLGAGPAPLLQGLLRPLKAQALPVLCRGLAVEAKKTYVRDKPHVNV GTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAAR HYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYV NKADAVQDSEMVELVELEIRELLTEFGYKGEETPIIVGSALCALEQRDPELGLKSVQKLL DAVDTYIPVPTRDLEKPFLLPVESVYSIPGRGTVVTGT*278LERGILKKGDECEFLGHSKNIR TVVTGIEMFHKS*312LDRAEAGDNLGALVRGLKREDLRRGLVMAKPGSIQPHQKVEAQVYILT KEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPGKELAMPGEDLKLTLILRQPMILEKGQ RFTLRDGNRTIGTGLVTDTPAMTEEDKNIKWS |
| SCOP | Class : Alpha and beta proteins (a/b) Fold : G domain-like Superfamily : Ras-like P-loop GTPases Family : Translation factor common region-like Domain Name : 1D2E A:55-343 Class : All beta proteins Fold : RIFT-type beta(6)-barrel Superfamily : EF-Tu post-G domain-like Family : Translation factor common region-like Domain Name : 1D2E A:55-343 |
| CATH | Matched CATH superfamily 2.40.30.10 3.40.50.300 |
| Predicted Disorder Regions | 1 predicted segment; (442-452) |
| DisProt Annotation | |
| TM Helix Prediction | No TM helices |
| PDB ID | 1D2E,1XB2, |
| Bibliography | Girodat D, Blanchard SC, Wieden HJ, Sanbonmatsu KY. Elongation Factor Tu Switch I Element is a Gate for Aminoacyl-tRNA Selection. J Mol Biol. 2020 Apr 17;432(9):3064-3077. doi: 10.1016/j.jmb.2020.01.038. Epub 2020 Feb 13. PMID: 32061931; PMCID: PMC8259901. |