Primary Information
BoMiProt ID	Bomi5421
Protein Name	EF-hand domain-containing protein D1/EF-hand domain-containing protein 1/Swiprosin-2
Organism	Bos taurus
Uniprot ID	Q17QM6
Milk Fraction	Exosomes
Ref Sequence ID	NP_001069300.1
Aminoacid Length	236
Molecular Weight	26540
FASTA Sequence	Download
Gene Name	EFHD1
Gene ID	522462
Protein Existence Status	Reviewed
Secondary Information
Presence in other biological fluids/tissue/cells	trachea
Protein Function	EFhd1, an actin-binding and -bundling protein in the mitochondria, may contribute to the Ca2+-dependent regulation of mitochondrial morphology and energy synthesis.
Biochemical Properties	The EF-hand domain-containing protein 1 (EFhd1), a homologous protein of EFhd2 has Ca2+-binding EF-hand motifs.The canonical EF-hand domain has two helix-loop-helix motifs comprising four helices (helix 1, helix 2, helix 3, and helix 4) and forms two hydrophobic clusters (I and II) (Denessiouk et al., 2014). Helices 1 and 4 form hydrophobic cluster I, comprising three aromatic residues, and helices 2 and 3 usually form hydrophobic cluster II, comprising a combination of aromatic, hydrophobic, and polar amino acids.
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	NA
Predicted Disorder Regions	1-53, 228-236
DisProt Annotation
TM Helix Prediction	No TM helices
Bibliography	1.Mun SA, Park J, Park KR, Lee Y, Kang JY, Park T, Jin M, Yang J, Jun CD, Eom SH. Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria. Front Cell Dev Biol. 2021 Jan 18;8:628222. doi: 10.3389/fcell.2020.628222. PMID: 33537316; PMCID: PMC7848108. 2.Two structural motifs within canonical EF-hand calcium-binding domains identify five different classes of calcium buffers and sensors.Denessiouk K, Permyakov S, Denesyuk A, Permyakov E, Johnson MS.PLoS One. 2014; 9(10):e109287. 3.Molecular characterization of mitocalcin, a novel mitochondrial Ca2+-binding protein with EF-hand and coiled-coil domains.Tominaga M, Kurihara H, Honda S, Amakawa G, Sakai T, Tomooka Y.J Neurochem. 2006 Jan; 96(1):292-304. 4.Fraternal twins: Swiprosin-1/EFhd2 and Swiprosin-2/EFhd1, two homologous EF-hand containing calcium binding adaptor proteins with distinct functions.Dütting S, Brachs S, Mielenz D.Cell Commun Signal. 2011 Jan 18; 9():2.