Search by BoMiProt ID - Bomi5395

Primary Information

BoMiProt ID Bomi5395
Protein Name Early growth response protein 1
Organism Bos taurus
Uniprot IDQ29W20
Milk FractionWhey
Ref Sequence ID NP_001039340.1
Aminoacid Length 540
Molecular Weight 57115
FASTA Sequence Download
Gene Name EGR1
Gene ID 407125
Protein Existence Status reviewed

Secondary Information

Protein Function EGR1 affects synaptic plasticity through regulation of target genes contributing to higher order processes such as learning and memory. The EGR1–YAP-1 interaction is important for the regulation of BCL2 associated X apoptosis regulator (BAX) expression, playing a crucial role in EGR1-mediated apoptosisIt is a transcriptional regulator which may be involved in recognizing and binding with the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes.
Biochemical Properties The modular structure of the EGR1 protein consists of a strong N-terminal and a weak C-terminal activation domain, an inhibitory domain, a DNA-binding module composed of three zinc-finger domains, and a bipartite nuclear localization signal.The inhibitory domain between the N-terminal activation domain and the DNA-binding domain binds two transcriptional co-factors termed NGFI-A binding proteins 1 and 2 (NAB1, NAB2) that block the biological activity of EGR1.
Significance in milk potential involvement in the regulation of preovulatory events in cattle.
PTMs Ubl conjugation,Phosphorylation,SUMOylation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions (21-111), (167-219), (268-280), (418-429)
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs  EGR1 can be sumoylated by the Ubiquitin conjugating enzyme (Ubc9)/Small ubiquitin-related modifier 1 (SUMO-1)/Cyclin dependent kinase inhibitor 2A (ARF) system by a mechanism dependent on the phosphorylation by protein kinase B (AKT).The sumoylation of EGR1 may be involved in its ubiquitination and degradation, thereby affecting protein stability. The hyperphosphorylation of EGR1 by the protein kinase, casein kinase II (CKII), has a negative effect on its DNA-binding and transcriptional activity.
Bibliography 1.Aliperti, V., Sgueglia, G., Aniello, F., Vitale, E., Fucci, L., & Donizetti, A. (2019). Identification, Characterization, and Regulatory Mechanisms of a Novel EGR1 Splicing Isoform. International journal of molecular sciences, 20(7), 1548. 2.Zhang, W. W., Shao, S. L., Pan, Y., & Li, S. S. (2019). Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, 35(1), 5–8.