Primary Information |
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| BoMiProt ID | Bomi5395 |
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| Protein Name | Early growth response protein 1 |
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| Organism | Bos taurus |
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| Uniprot ID | Q29W20 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001039340.1 |
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| Aminoacid Length | 540 |
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| Molecular Weight | 57115 |
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| FASTA Sequence |
Download |
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| Gene Name | EGR1 |
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| Gene ID | 407125 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | EGR1 affects synaptic plasticity through regulation of target genes contributing to higher order processes such as learning and memory. The EGR1–YAP-1 interaction is important for the regulation of BCL2 associated X apoptosis regulator (BAX) expression, playing a crucial role in EGR1-mediated apoptosisIt is a transcriptional regulator which may be involved in recognizing and binding with the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. |
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| Biochemical Properties | The modular structure of the EGR1 protein consists of a strong N-terminal and a weak C-terminal activation domain, an inhibitory domain, a DNA-binding module composed of three zinc-finger domains, and a bipartite nuclear localization signal.The inhibitory domain between the N-terminal activation domain and the DNA-binding domain binds two transcriptional co-factors termed NGFI-A binding proteins 1 and 2 (NAB1, NAB2) that block the biological activity of EGR1. |
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| Significance in milk | potential involvement in the regulation of preovulatory events in cattle. |
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| PTMs | Ubl conjugation,Phosphorylation,SUMOylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| NA |
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| Predicted Disorder Regions | (21-111), (167-219), (268-280), (418-429) |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | EGR1 can be sumoylated by the Ubiquitin conjugating enzyme (Ubc9)/Small ubiquitin-related modifier 1 (SUMO-1)/Cyclin dependent kinase inhibitor 2A (ARF) system by a mechanism dependent on the phosphorylation by protein kinase B (AKT).The sumoylation of EGR1 may be involved in its ubiquitination and degradation, thereby affecting protein stability. The hyperphosphorylation of EGR1 by the protein kinase, casein kinase II (CKII), has a negative effect on its DNA-binding and transcriptional activity. |
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| Bibliography | 1.Aliperti, V., Sgueglia, G., Aniello, F., Vitale, E., Fucci, L., & Donizetti, A. (2019). Identification, Characterization, and Regulatory Mechanisms of a Novel EGR1 Splicing Isoform. International journal of molecular sciences, 20(7), 1548. https://doi.org/10.3390/ijms20071548 2.Zhang, W. W., Shao, S. L., Pan, Y., & Li, S. S. (2019). Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, 35(1), 5–8. https://doi.org/10.12047/j.cjap.5709.2019.002 |
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