Primary Information |
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| BoMiProt ID | Bomi5374 |
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| Protein Name | E3 ubiquitin-protein ligase RNF25 |
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| Organism | Bos taurus |
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| Uniprot ID | Q5E9N3 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001015575.1 |
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| Aminoacid Length | 458 |
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| Molecular Weight | 51236 |
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| FASTA Sequence |
Download |
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| Gene Name | RNF25 |
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| Gene ID | 511571 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | is an E3 ubiquitin ligase targets Nkd2 for degradation,physically interacts with Nkd1 and Axin in an E3 ligase-independent manner to strengthen Wnt signalling.Stimulates transcription mediated by NF-kappa-B and EGF Receptor (EGFR) signaling. |
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| Biochemical Properties | The interaction with Nkd2 is mediated by the RING domain (aa 113–291), a domain that is essential for its E3 ubiquitin ligase activity, leading to Nkd2 polyubiquitination and degradation.Also C-terminal Proline rich region (aa 292–513) is imp for interaction. |
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| PTMs | Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| NA |
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| Predicted Disorder Regions | 1-7, 174-189, 218-332, 351-375, 397-406, 452-458 |
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| DisProt Annotation | |
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| TM Helix Prediction | no TM helices |
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| Bibliography | 1.Gao R, Ma LQ, Du X, Zhang TT, Zhao L, Liu L, Liu JC, Guo F, Cheng Z, Huang H. Rnf25/AO7 positively regulates wnt signaling via disrupting Nkd1-Axin inhibitory complex independent of its ubiquitin ligase activity. Oncotarget. 2016 Apr 26;7(17):23850-9. doi: 10.18632/oncotarget.8126. PMID: 27007149; PMCID: PMC5029668. |
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