Primary Information |
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| BoMiProt ID | Bomi5192 |
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| Protein Name | DNA polymerase alpha subunit B/DNA polymerase alpha 70 kDa subunit |
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| Organism | Bos taurus |
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| Uniprot ID | Q58D13 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001019713.2 |
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| Aminoacid Length | 604 |
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| Molecular Weight | 66955 |
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| FASTA Sequence |
Download |
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| Gene Name | POLA2 |
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| Gene ID | 514793 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | subunit of DNA polymerase alpha complex associates with primase and forms a Polalpha-primase complex,which plays an imp function in replication initiation.Responsible for both initiation of both leading and lagging strands at the replication origins.The p49 is the primase catalytic subunit that synthesizes the RNA primer. |
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| PTMs | Phosphorylation on Ser and Thr |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q58D13|DPOA2_BOVIN DNA polymerase alpha subunit B OS=Bos taurus OX=9913 GN=POLA2 PE=2 SV=1
MAVSAQLLVEELQIFGLECEEAVIEKLVELCILYGQNEEGMASELIAFCTSTRKDCFTLE
TLNSFEHEFLSKRVSKTRHGASKDKGLRHAGARDIVSIQELIEVEEEEETLLNSYTTPSK
GSQKRTITT*129PET*132PLTKRSVSARS*143PHQLLS*149PSSFS*154PS*156ATPPQKYSSRSNRGEVVTSFGSAQGVSWSGRGGASPLSLKVLGHPEPLTGSYKYMFQKLPDIREVLTCKIEELGSELKEHYKIE
AFAPILVPAQEPVTLLGQIGCDSNGKLNHKSVILEGDLEHSSGAQIPVDLSELKEYSLFP
GQVVVMEGINTTGRKLVATRLYEGVPLPFHQPDEEDGDSEQFMVLVACGPYTTSDSITFD
PLLDLITIINRDRPDVCILFGPFLDAKHEQVESCLLTSSFEDVFKQCLRTIIEGTRSSGS
HLIIVPSLRDVHHEPVYPQPPFSCSDLLREDKKRVRLVSEPCTLSINGVIFGLTSTDLLF
HMGAEEISSSSGTSDRFSRILRHILTQRSYYPLYPPQEDMAIDYENFYLYAQLPVTPDVF
IAPSELRYFVKVGLNSAISLQCQNQFLSGALALNSSRVCWWGQWPRSFGGGVGVENKSVS
IMLK
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| Predicted Disorder Regions | 75-169 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | Phosphorylated in a cell cycle-dependent manner, in G2/M phase.The p70(catalytic) subunit is phosphorylated only in G2/M phase.p180 is 5 times more highly phosphorylated. |
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| Bibliography | 1.Nasheuer HP, Moore A, Wahl AF, Wang TS. Cell cycle-dependent phosphorylation of human DNA polymerase alpha. J Biol Chem. 1991 Apr 25;266(12):7893-903. PMID: 1902230. 2.Uchiyama M, Wang TS. The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication. Mol Cell Biol. 2004 Sep;24(17):7419-34. doi: 10.1128/MCB.24.17.7419-7434.2004. PMID: 15314153; PMCID: PMC506996. |
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