Search by BoMiProt ID - Bomi4991

Primary Information

BoMiProt ID Bomi4991
Protein Name Cytochrome c1, heme protein, mitochondrial/Complex III subunit 4/Complex III subunit IV/Cytochrome b-c1 complex subunit 4/Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit/Cytochrome c-1
Organism Bos taurus
Uniprot IDP00125
Milk FractionWhey
Ref Sequence ID NP_001033179.1
Aminoacid Length 325
Molecular Weight 35297
FASTA Sequence Download
Gene Name CYC1
Gene ID 512500
Protein Existence Status reviewed

Secondary Information

Protein Function Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.Cytochrome c1 also forms a ternary complex with cytochrome c and oxidase in the heme ratio of 1:1:1.  
Biochemical Properties Cofactor is heme.Binds 1 heme group covalenty per subunit.Complex III catalyzes the transport of reducing equivalents from CoQ to cytochrome c.The NH2- and COOH-terminal residues are serine and lysine, respectively.46% a-helix and 15% P-sheet in bovine Complex c1
PTMs phosphorylation,Acetylation,Nitration,Trimethylation,Nitrosylation,Sulfoxidation.
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
SCOP Class : All alpha proteins
Fold : Cytochrome c
Superfamily : Cytochrome c
Family : Cytochrome bc1 domain
Domain Name : 1PPJ D:1-195

Class : All alpha proteins
Fold : Single transmembrane helix
Superfamily : Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor
Family : Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor
Domain Name : 1PPJ D:196-241

CATH Matched CATH superfamily
Predicted Disorder Regions 1-4, 40-58, 151-189, 311-325
DisProt Annotation
TM Helix Prediction 1TMH; (288-306)
Significance of PTMs In mouse liver mitochondria,Acetylation on Lys8.Phosphorylation on Thr28 in Bovine kidney is associated with lower respiration, membrane potential, and human muscle,Ser47 phosphorylation associated with lower caspase activity.Tyr 48 phosphorylation in Bovine liver is associated with Lower COX activity, abolished caspase-3 activity, and lower cardiolipin peroxidase activity.Nitration at Tyr67 in Horse heart is associated with reduced mitochondrial yeast Trimethylation on Lys72 is associated with Cytc import into the mitochondria, prevents apoptosis, and improves protein stability against proteolytic degradation.Nitration Tyr74 is associated with Increased cardiolipin peroxidase activity and suppressed caspase-9 Horse heart,Nitrosylation and Sulfoxidation at Met80 is associated with Increased cardiolipin peroxidase activity.In Bovine heart,there is phosphorylation on Tyr 97 which is associated with Lower COX activity; reduced Cytc release and apoptosis after ischemia/reperfusion when treated with insulin.
Bibliography 1.Wakabayashi S, Matsubara H, Kim CH, King TE. Structural studies of bovine heart cytochrome c1. J Biol Chem. 1982 Aug 25;257(16):9335-44. PMID: 6286615. 2.Kalpage HA, Bazylianska V, Recanati MA, Fite A, Liu J, Wan J, Mantena N, Malek MH, Podgorski I, Heath EI, Vaishnav A, Edwards BF, Grossman LI, Sanderson TH, Lee I, Hüttemann M. Tissue-specific regulation of cytochrome c by post-translational modifications: respiration, the mitochondrial membrane potential, ROS, and apoptosis. FASEB J. 2019 Feb;33(2):1540-1553. doi: 10.1096/fj.201801417R. Epub 2018 Sep 17. PMID: 30222078; PMCID: PMC6338631.