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Primary Information | |
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| BoMiProt ID | Bomi4991 |
| Protein Name | Cytochrome c1, heme protein, mitochondrial/Complex III subunit 4/Complex III subunit IV/Cytochrome b-c1 complex subunit 4/Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit/Cytochrome c-1 |
| Organism | Bos taurus |
| Uniprot ID | P00125 |
| Milk Fraction | Whey |
| Ref Sequence ID | NP_001033179.1 |
| Aminoacid Length | 325 |
| Molecular Weight | 35297 |
| FASTA Sequence | Download |
| Gene Name | CYC1 |
| Gene ID | 512500 |
| Protein Existence Status | reviewed |
Secondary Information | |
| Protein Function | Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.Cytochrome c1 also forms a ternary complex with cytochrome c and oxidase in the heme ratio of 1:1:1. |
| Biochemical Properties | Cofactor is heme.Binds 1 heme group covalenty per subunit.Complex III catalyzes the transport of reducing equivalents from CoQ to cytochrome c.The NH2- and COOH-terminal residues are serine and lysine, respectively.46% a-helix and 15% P-sheet in bovine Complex c1 |
| PTMs | phosphorylation,Acetylation,Nitration,Trimethylation,Nitrosylation,Sulfoxidation. |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | na |
| SCOP | Class : All alpha proteins Fold : Cytochrome c Superfamily : Cytochrome c Family : Cytochrome bc1 domain Domain Name : 1PPJ D:1-195 Class : All alpha proteins Fold : Single transmembrane helix Superfamily : Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor Family : Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor Domain Name : 1PPJ D:196-241 |
| CATH | Matched CATH superfamily 1.10.760.10 1.20.5.10 1.20.5.100 |
| Predicted Disorder Regions | 1-4, 40-58, 151-189, 311-325 |
| DisProt Annotation | |
| TM Helix Prediction | 1TMH; (288-306) |
| Significance of PTMs | In mouse liver mitochondria,Acetylation on Lys8.Phosphorylation on Thr28 in Bovine kidney is associated with lower respiration, membrane potential, and ROS.in human muscle,Ser47 phosphorylation associated with lower caspase activity.Tyr 48 phosphorylation in Bovine liver is associated with Lower COX activity, abolished caspase-3 activity, and lower cardiolipin peroxidase activity.Nitration at Tyr67 in Horse heart is associated with reduced mitochondrial respiration.in yeast Trimethylation on Lys72 is associated with Cytc import into the mitochondria, prevents apoptosis, and improves protein stability against proteolytic degradation.Nitration Tyr74 is associated with Increased cardiolipin peroxidase activity and suppressed caspase-9 activity.in Horse heart,Nitrosylation and Sulfoxidation at Met80 is associated with Increased cardiolipin peroxidase activity.In Bovine heart,there is phosphorylation on Tyr 97 which is associated with Lower COX activity; reduced Cytc release and apoptosis after ischemia/reperfusion when treated with insulin. |
| PDB ID | 1BCC,1BE3,1BGY,1L0L,1L0N,1NTK,1NTM,1NTZ,1NU1,1PP9,1PPJ,1QCR,1SQB,1SQP,1SQQ,1SQV,1SQX,2A06,2BCC,2FYU,2YBB,3BCC,4D6T,4D6U,5GPN,5KLV,5LUF,5NMI, 5OKD,6FO0,6FO2,6FO6,6HAW,6NHG,6XVF,6ZFS, 6ZFT,6ZFU, |
| Bibliography | 1.Wakabayashi S, Matsubara H, Kim CH, King TE. Structural studies of bovine heart cytochrome c1. J Biol Chem. 1982 Aug 25;257(16):9335-44. PMID: 6286615. 2.Kalpage HA, Bazylianska V, Recanati MA, Fite A, Liu J, Wan J, Mantena N, Malek MH, Podgorski I, Heath EI, Vaishnav A, Edwards BF, Grossman LI, Sanderson TH, Lee I, Hüttemann M. Tissue-specific regulation of cytochrome c by post-translational modifications: respiration, the mitochondrial membrane potential, ROS, and apoptosis. FASEB J. 2019 Feb;33(2):1540-1553. doi: 10.1096/fj.201801417R. Epub 2018 Sep 17. PMID: 30222078; PMCID: PMC6338631. |