Primary Information
BoMiProt ID	Bomi4976
Protein Name	Cysteine--tRNA ligase, mitochondrial/Cysteinyl-tRNA synthetase/CysRS
Organism	Bos taurus
Uniprot ID	Q2KIF8
Milk Fraction	Whey
Aminoacid Length	555
Molecular Weight	61199
FASTA Sequence	Download
Gene Name	CARS2
Gene ID	506598
Protein Existence Status	reviewed
Secondary Information
Protein Function	involved in mitochondrial biogenesis and bioenergetics via CysSSH(Cysteine hydropersulfide) production,which are essential structural components of several proteins and enzymes, e.g. serving as metal ligands in iron-sulfur clusters (or sulfide donors) and in iron-cysteine and zinc-cysteine complex.
Biochemical Properties	Binds 1 zinc ion per subunit.Recognizes tRNA-Cys and utilizes ATP as cofactor and L-Cysteine as substrate to form L-cysteinyl-tRNA-Cys.
Significance in milk	hydrolyzing casein
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	na
Predicted Disorder Regions	NA
DisProt Annotation
TM Helix Prediction	No TM helices
Bibliography	Akaike T, Ida T, Wei FY, Nishida M, Kumagai Y, Alam MM, Ihara H, Sawa T, Matsunaga T, Kasamatsu S, Nishimura A, Morita M, Tomizawa K, Nishimura A, Watanabe S, Inaba K, Shima H, Tanuma N, Jung M, Fujii S, Watanabe Y, Ohmuraya M, Nagy P, Feelisch M, Fukuto JM, Motohashi H. Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics. Nat Commun. 2017 Oct 27;8(1):1177. doi: 10.1038/s41467-017-01311-y. PMID: 29079736; PMCID: PMC5660078.