Search by BoMiProt ID - Bomi4863

Primary Information

BoMiProt ID Bomi4863
Protein Name Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'/cGMP phosphodiesterase 6C/PDE V-C1
Organism Bos taurus
Uniprot IDP16586
Milk Fractionwhey
Ref Sequence ID NP_776844.1
Aminoacid Length 855
Molecular Weight 98798
FASTA Sequence Download
Gene Name PDE6C/PDEA2
Gene ID 281975
Protein Existence Status Reviewed

Secondary Information

Protein Function It acts as cone-specific cGMP phosphodiesterase, it plays an essential role in light detection and cone phototransduction by rapidly decreasing intracellular levels of cGMP
Biochemical Properties Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.Catalyzes the following reaction-3',5'-cyclic GMP + H2O = GMP + H+. Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.Contain a C-terminal isoprenylation motif to bind the prenyl binding protein/δ (PDE6D).The active site of PDE6C shows a very similar selectivity to the rod PDE6 enzyme for inhibition of catalysis by phosphodiesterase inhibitors.
PTMs Prenylation and Methylation,Lipidation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
CATH Matched CATH superfamily
Predicted Disorder Regions (821-855)
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs PDE6C contains a carboxyl-terminal CaaX sequence motif that is predicted to direct this protein to undergo sequential posttranslational processing. The first step is the addition of a prenyl moiety on the cysteine residue that is four amino acids from the C-terminus. Next, an endoproteolytic cleavage removes the last three amino acid residues and the prenylated cysteine becomes carboxymethylated. All mammalian PDE6C proteins contain a terminal leucine residue within the CaaX motif making it the preferred substrate for geranylgeranyl transferase (GGTase-I). After biosynthesis, prenylated PDE6C dock to the endoplasmic reticulum and is trafficked by vesicular transport to the outer segments.In zebrafish, a mutation in the PDE6C gene results in a developmental progression of retinal degeneration similar to that of the rd1 mouse model (a model for rod photoreceptor degenerative diseases. Mutations in the PDE6C gene that correlate with diseases such as cone dystrophy and achromatopsia. Defective transport of prenylated proteins (including PDE6C) is observed in mice that lack the prenyl binding protein-δ (PrBP/δ; PDE6D) gene, resulting in altered photoreceptor physiology and slowly progressing cone dystrophy.
Bibliography 1.Cahill KB, Cote RH. Phosphodiesterase 6C, cGMP-specific cone alpha'. AFCS Nat Mol Pages. 2011;2011:A001756. doi: 10.1038/mp.a001756.01. Epub 2011 Jun 3. PMID: 26504430; PMCID: PMC4618378. 2.Li, T. S., Volpp, K., & Applebury, M. L. (1990). Bovine cone photoreceptor cGMP phosphodiesterase structure deduced from a cDNA clone. Proceedings of the National Academy of Sciences of the United States of America, 87(1), 293–297.